Anti-HOXD10 antibody (ab86014)
Key features and details
- Rabbit polyclonal to HOXD10
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-HOXD10 antibody
See all HOXD10 primary antibodies -
Description
Rabbit polyclonal to HOXD10 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Recombinant fragment
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Immunogen
Three synthetic peptides (human) conjugated to KLH.
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Positive control
- Induced culture of E. coli
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate) -
Concentration information loading... -
Purity
Whole antiserum -
Purification notes
Crude serum, final bleed. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HOXD10 antibody (ab86014)All lanes : Anti-HOXD10 antibody (ab86014) at 1/1000 dilution (in 5% non fat milk PBST blocking solution)
Lane 1 : Uninduced culture (plus anti thio at 1/5000 dilution)
Lane 2 : Induced culture of E. coli (plus anti thio at 1/5000 dilution)
Secondary
All lanes : anti-Rabbit IgG HRP at 1/1000 dilution (in 5% non fat milk PBST blocking solution)
Predicted band size: 38 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Exposure time: 1 hour
Plasmid name: pBAD-DEST49.
The actual band size differs from the predicted size because it depicts HOXD10 (38.4 kDa) plus a tag (14 kDa)
