All lanes : Anti-HOXA13 antibody (ab106503) at 1 µg/ml

Lane 1 : HEK293 (Human) Whole Cell Lysate (ab52256)
Lane 2 : HEK293 (Human) Whole Cell Lysate (ab52256) with Immunising peptide at 1 µg/ml

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 40 kDa
Observed band size: 40 kDa
Additional bands at: 73 kDa. We are unsure as to the identity of these extra bands.


Exposure time: 8 minutes

IHC image of ab106503 staining in human skin formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab106503, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.