Anti-HO-2 antibody (ab245605)
Key features and details
- Rabbit polyclonal to HO-2
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HO-2 antibody
See all HO-2 primary antibodies -
Description
Rabbit polyclonal to HO-2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Human
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Immunogen
Synthetic peptide within Human HO-2 aa 1-50. The exact sequence is proprietary.
Database link: P30519 -
Positive control
- IP: HO-2 IP in HEK-293T whole cell lysate.
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General notes
ab245605 has not performed satisfactorily when used for WB of HO-2 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of HO-2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab245605 was affinity purified using an epitope specific to HO-2 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-HO-2 antibody (ab245605)
HO-2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) using ab245605 at 6 μg/reaction. Western blot was performed on the immunoprecipitate using ab245605 at 1 μg/ml.
Lane 1: ab245605 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG in HEK-293T whole cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
