Anti-HNF-4-alpha (phospho S313) antibody (ab78356)
Key features and details
- Rabbit polyclonal to HNF-4-alpha (phospho S313)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HNF-4-alpha (phospho S313) antibody
See all HNF-4-alpha primary antibodies -
Description
Rabbit polyclonal to HNF-4-alpha (phospho S313) -
Host species
Rabbit -
Specificity
This antibody detects endogenous levels of HNF-4-alpha only when phosphorylated at serine 313. -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide corresponding to Human HNF-4-alpha. Synthesized phosphopeptide derived from human HNF-4-alpha around the phosphorylation site of serine 313 (L-R-SP-Q-V).
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Positive control
- Extracts from HUVEC cells. Human liver carcinoma tissue. LOVO cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
- Epigenetics and Nuclear Signaling
- Transcription
- Polymerase associated factors
- Pol II Transcription
- Other
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipases
Images
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Western blot - Anti-HNF-4-alpha (phospho S313) antibody (ab78356)All lanes : Anti-HNF-4-alpha (phospho S313) antibody (ab78356) at 1/500 dilution
Lane 1 : Extracts from HUVEC cells,
treated with EGF (200ng/ml, 30mins)
Lane 2 : Extracts from HUVEC cells,
treated with EGF (200ng/ml, 30mins) with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 54 kDa
Observed band size: 54 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha (phospho S313) antibody (ab78356)Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using ab78356 at a 1/50 dilution.
Left image un-treated.
Right image treated with immunizing peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-HNF-4-alpha (phospho S313) antibody (ab78356)Immunofluorescence analysis of LOVO cells using ab78356 at a 1/500 dilution.
Left image un-treated.
Right image treated with immunizing peptide.
