Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HNF-4-alpha antibody [EPR16787] - BSA and Azide free (ab251313)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16787] to HNF-4-alpha - BSA and Azide free
  • Suitable for: ICC, WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-HNF-4-alpha antibody [EPR16787] - BSA and Azide free
    See all HNF-4-alpha primary antibodies

  • Description

    Rabbit monoclonal [EPR16787] to HNF-4-alpha - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251313 is the carrier-free version of ab200654. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251313 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Western blot - Anti-HNF-4-alpha antibody [EPR16787] - BSA and Azide free (ab251313)
    Western blot - Anti-HNF-4-alpha antibody [EPR16787] - BSA and Azide free (ab251313)
    All lanes : Anti-HNF-4-alpha antibody [EPR16787] (ab200654) at 1/10000 dilution

    Lane 1 : SW480 (Human colon adenocarcinoma cell line) whole cell lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 50 kDa
    Observed band size: 50 kDa


    Exposure time: 3 minutes


    This data was developed using ab200654, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-HNF-4-alpha antibody [EPR16787] - BSA and Azide free (ab251313)
    Western blot - Anti-HNF-4-alpha antibody [EPR16787] - BSA and Azide free (ab251313)
    Anti-HNF-4-alpha antibody [EPR16787] (ab200654) at 1/1000 dilution + Human colon cancer tissue lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 50 kDa
    Observed band size: 50 kDa


    Exposure time: 3 minutes


    This data was developed using ab200654, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16787] - BSA and Azide free (ab251313)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16787] - BSA and Azide free (ab251313)
    This data was developed using ab200654, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma tissue labeling HNF-4-alpha with ab200654 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear staining on Human colonic carcinoma tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16787] - BSA and Azide free (ab251313)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16787] - BSA and Azide free (ab251313)
    This data was developed using ab200654, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling HNF-4-alpha with ab200654 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear staining on Human hepatocellular carcinoma tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-HNF-4-alpha antibody [EPR16787] - BSA and Azide free (ab251313)
    Immunocytochemistry - Anti-HNF-4-alpha antibody [EPR16787] - BSA and Azide free (ab251313)
    This data was developed using ab200654, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling HNF-4-alpha with ab200654 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).Nuclear and weakly cytoplasmic staining on HepG2 cell line is observed.The nuclear counter stain is DAPI (blue).Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab200654 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
  • Anti-HNF-4-alpha antibody [EPR16787] - BSA and Azide free (ab251313)
    Anti-HNF-4-alpha antibody [EPR16787] - BSA and Azide free (ab251313)

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