Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Histone H3 (ubiquityl K23) antibody [EPR18876] - BSA and Azide free (ab251182)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18876] to Histone H3 (ubiquityl K23) - BSA and Azide free
  • Suitable for: Dot blot, WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Histone H3 (ubiquityl K23) antibody [EPR18876] - BSA and Azide free
    See all Histone H3 primary antibodies

  • Description

    Rabbit monoclonal [EPR18876] to Histone H3 (ubiquityl K23) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Dot
    Recombinant fragment
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251182 is the carrier-free version of ab194243. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251182 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Clonality

    Monoclonal

  • Clone number

    EPR18876

  • Isotype

    IgG

Images

  • Western blot - Anti-Histone H3 (ubiquityl K23) antibody [EPR18876] - BSA and Azide free (ab251182)
    Western blot - Anti-Histone H3 (ubiquityl K23) antibody [EPR18876] - BSA and Azide free (ab251182)
    All lanes : Anti-Histone H3 (ubiquityl K23) antibody [EPR18876] (ab194243) at 1/20000 dilution

    Lane 1 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : Whole cell lysate from HeLa treated with 2mM thymidine for 18 hours, followed by addition of fresh culture medium and incubation for 9 hours. Cell were then treated with 2mM thymidine for 15 hours, followed by addition of fresh culture medium and incubation for 4 hours

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 15 kDa
    Observed band size: 28 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab194243, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The expression level of H3K23ubi is increased during S phase.

    The MW is consistent with literature (PMID: 24013172).

  • Immunoprecipitation - Anti-Histone H3 (ubiquityl K23) antibody [EPR18876] - BSA and Azide free (ab251182)
    Immunoprecipitation - Anti-Histone H3 (ubiquityl K23) antibody [EPR18876] - BSA and Azide free (ab251182)
    This data was developed using ab194243, the same antibody clone in a different buffer formulation.Histone H3 (ubiquityl K23) was immunoprecipitated using ab194243 at 1/30 dilution from 0.35 mg of whole cell lysate from HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with 2mM thymidine for 18 hours, followed by addition of fresh culture medium and incubation for 9 hours, then treated with 2mM thymidine for 15 hours, followed by addition of fresh culture medium and incubation for 4 hours. Western blot was performed from the immunoprecipitate using ab194243 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: 10 µg of whole cell lysate from HeLa treated with 2mM thymidine for 18 hours, followed by addition of fresh culture medium and incubation for 9 hours, cells were then treated with 2mM thymidine for 15 hours, followed by addition of fresh culture medium and incubation for 4 hours (Input). Lane 2: ab194243 IP in whole cell lysate from HeLa treated with 2mM thymidine for 18 hours, followed by addition of fresh culture medium and incubation for 9 hours, cells were then treated with 2mM thymidine for 15 hours, followed by addition of fresh culture medium and incubation for 4 hours. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab194243 in whole cell lysate from HeLa treated with 2mM thymidine for 18 hours, followed by addition of fresh culture medium and incubation for 9 hours, cells were then treated with 2mM thymidine for 15 hours, followed by addition of fresh culture medium and incubation for 4 hours. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 3 seconds. The band at around 35kDa can be explained as di-ubiquitinated K23 (PMID24013172).
  • Western blot - Anti-Histone H3 (ubiquityl K23) antibody [EPR18876] - BSA and Azide free (ab251182)
    Western blot - Anti-Histone H3 (ubiquityl K23) antibody [EPR18876] - BSA and Azide free (ab251182)
    All lanes : Anti-Histone H3 (ubiquityl K23) antibody [EPR18876] (ab194243) at 1/20000 dilution

    Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg
    Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 15 kDa
    Observed band size: 28 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab194243, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The MW is consistent with literature: PMID 24013172.

  • Dot Blot - Anti-Histone H3 (ubiquityl K23) antibody [EPR18876] - BSA and Azide free (ab251182)
    Dot Blot - Anti-Histone H3 (ubiquityl K23) antibody [EPR18876] - BSA and Azide free (ab251182)
    This data was developed using ab194243, the same antibody clone in a different buffer formulation.Dot blot analysis of Histone H3 (ubiquityl K23) labeled with ab194243 at 1/1000 dilution. Lane 1: Histone H3 (ubiquitinated K23) peptide (aa20-29). Lane 2: Histone H3 non- ubiquitinated peptide (aa22-31). Lane 3: Histone H3 full length recombinant protein. Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 was used as secondary antibody. Blocking and diluting buffer: 5% NFDM/TBST. Exposure time: 3 minutes.
  • Anti-Histone H3 (ubiquityl K23) antibody [EPR18876] - BSA and Azide free (ab251182)
    Anti-Histone H3 (ubiquityl K23) antibody [EPR18876] - BSA and Azide free (ab251182)

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