Anti-Histone H3 (tri methyl K9, phospho S10) antibody [RM162] (ab238306)
![Anti-Histone H3 (tri methyl K9, phospho S10) antibody [RM162] (ab238306)](https://www.abcam.com/ps/products/238/ab238306/Images/ab238306-316107-anti-histone-h3-tri-methyl-k9-phospho-s10-antibody-rm162-western-blot.png "Anti-Histone H3 (tri methyl K9, phospho S10) antibody [RM162] (ab238306)")
Key features and details
- Rabbit monoclonal [RM162] to Histone H3 (tri methyl K9, phospho S10)
- Suitable for: ELISA, WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (tri methyl K9, phospho S10) antibody [RM162]
See all Histone H3 primary antibodies -
Description
Rabbit monoclonal [RM162] to Histone H3 (tri methyl K9, phospho S10) -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Synthetic peptide corresponding to Human Histone H3 (tri methyl K9, phospho S10).
Database link: P84243 -
Positive control
- WB: Acid extract of HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RM162 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Histone H3 (tri methyl K9, phospho S10) antibody [RM162] (ab238306)All lanes : Anti-Histone H3 (tri methyl K9, phospho S10) antibody [RM162] (ab238306) at 0.01 µg/ml
Lane 1 : Acid extracts of HeLa (human epithelial cell line from cervix adenocarcinoma) cells
Lane 2 : Recombinant Histone H3.3
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![ELISA - Anti-Histone H3 (tri methyl K9, phospho S10) antibody [RM162] (ab238306)](https://www.abcam.com/ps/products/238/ab238306/Images/ab238306-316106-anti-histone-h3-tri-methyl-k9-phospho-s10-antibody-rm162-elisa.png)
ELISA - Anti-Histone H3 (tri methyl K9, phospho S10) antibody [RM162] (ab238306)ab238306 specifically reacts to Histone H3 only when modified by both trimethylation at lysine 9 and phosphorylation at serine 10 (K9me3/S10p).
No cross reactivity with non-modified Lysine 9/ Serine 10, methylated Lysine 9 (K9me1, k9me2, k9me3) ONLY, or phosphorylation at Serine 9 ONLY in Histone H3.
