Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Histone H3 (phospho T3) antibody [EP1702Y] - BSA and Azide free (ab247494)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1702Y] to Histone H3 (phospho T3) - BSA and Azide free
  • Suitable for: ICC, IHC-P, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Histone H3 (phospho T3) antibody [EP1702Y] - BSA and Azide free
    See all Histone H3 primary antibodies

  • Description

    Rabbit monoclonal [EP1702Y] to Histone H3 (phospho T3) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody detects Histone H3 phosphorylated at Theronine 3. Species cross reactivity was determined by western blot only. No signal was detected in the following Mouse and Rat tissues lysates: brain, heart, kidney, and spleen.

  • Tested applications

    Suitable for: ICC, IHC-P, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC: HeLa cells

  • General notes

    ab247494 is the carrier-free version of ab78351 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247494 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Affinity purified

  • Clonality

    Monoclonal

  • Clone number

    EP1702Y

  • Isotype

    IgG

Images

  • Western blot - Anti-Histone H3 (phospho T3) antibody [EP1702Y] - BSA and Azide free (ab247494)
    Western blot - Anti-Histone H3 (phospho T3) antibody [EP1702Y] - BSA and Azide free (ab247494)
    All lanes : Anti-Histone H3 (phospho T3) antibody [EP1702Y] (ab78351) at 1/500000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HeLa cell lysate treated with FBS + Calyculin A.

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 16 kDa
    Observed band size: 14 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab78351, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (phospho T3) antibody [EP1702Y] - BSA and Azide free (ab247494)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (phospho T3) antibody [EP1702Y] - BSA and Azide free (ab247494)
    This data was developed using ab78351, the same antibody clone in a different buffer formulation.ab78351 at 1/100 dilution staining Histone H3 in human breast carcinoma tissue, using a HRP/AP polymerized secondary antibody. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-Histone H3 (phospho T3) antibody [EP1702Y] - BSA and Azide free (ab247494)
    Immunocytochemistry - Anti-Histone H3 (phospho T3) antibody [EP1702Y] - BSA and Azide free (ab247494)
    This data was developed using the same antibody clone in a different buffer formulation (ab78351).
    Immunocytochemistry analysis of HeLa (human cervix adenocarcinoma epithelial cell) labeling Histone H3 with purified ab78351 at 1/250 dilution. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.18 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
    Negative control: PBS instead of the primary antibody.
  • Flow Cytometry - Anti-Histone H3 (phospho T3) antibody [EP1702Y] - BSA and Azide free (ab247494)
    Flow Cytometry - Anti-Histone H3 (phospho T3) antibody [EP1702Y] - BSA and Azide free (ab247494)
    This data was developed using ab78351, the same antibody clone in a different buffer formulation.Overlay histogram showing HeLa cells stained with ab78351 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab78351, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was a goat anti-rabbit DyLight® 488 (IgG; H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Anti-Histone H3 (phospho T3) antibody [EP1702Y] - BSA and Azide free (ab247494)
    Anti-Histone H3 (phospho T3) antibody [EP1702Y] - BSA and Azide free (ab247494)

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