Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HIP1 antibody [EPR10814] - BSA and Azide free (ab250453)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10814] to HIP1 - BSA and Azide free
  • Suitable for: ICC, IP, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-HIP1 antibody [EPR10814] - BSA and Azide free
    See all HIP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR10814] to HIP1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab188140)

  • Positive control

    • WB: HAP1, HCT-116, HeLa, Jurkat and A549 cell lysates. ICC: HeLa cells.

  • General notes

    Ab250453 is the carrier-free version of ab181238. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250453 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-HIP1 antibody [EPR10814] - BSA and Azide free (ab250453)
    Western blot - Anti-HIP1 antibody [EPR10814] - BSA and Azide free (ab250453)
    All lanes : Anti-HIP1 antibody [EPR10814] (ab181238) at 1/1000 dilution

    Lane 1 : wild-type HAP1 cell lysate
    Lane 2 : HIP1 knockout HAP1 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 116 kDa
    Observed band size: 116 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab181238).

    Lanes 1 - 3: Merged signal (red and green). Green - ab181238 observed at 116 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab181238 was shown to react with HIP1 in wild-type HAP1 cells in western blot with loss of signal observed in HIP1 knockout sample. Wild-type and HIP1 knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab181238 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry - Anti-HIP1 antibody [EPR10814] - BSA and Azide free (ab250453)
    Immunocytochemistry - Anti-HIP1 antibody [EPR10814] - BSA and Azide free (ab250453)
    This data was developed using ab181238, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) labeling HIP1 with ab181238 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
  • Western blot - Anti-HIP1 antibody [EPR10814] - BSA and Azide free (ab250453)
    Western blot - Anti-HIP1 antibody [EPR10814] - BSA and Azide free (ab250453)
    All lanes : Anti-HIP1 antibody [EPR10814] (ab181238) at 1/2000 dilution

    Lane 1 : HCT-116 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 116 kDa



    This data was developed using ab181238, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-HIP1 antibody [EPR10814] - BSA and Azide free (ab250453)
    Immunoprecipitation - Anti-HIP1 antibody [EPR10814] - BSA and Azide free (ab250453)
    This data was developed using ab181238, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from A549 cell lysate immunoprecipitated using ab181238 at 1/50 dilution.
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.
  • Anti-HIP1 antibody [EPR10814] - BSA and Azide free (ab250453)
    Anti-HIP1 antibody [EPR10814] - BSA and Azide free (ab250453)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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