Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸ 10 µl 90 460 ₸

Order now and get it on Wednesday March 17, 2021

Anti-HHLA2 antibody [EPR23563-120] (ab254367)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23563-120] to HHLA2
Suitable for: Flow Cyt
Reacts with: Human

Product name

Anti-HHLA2 antibody [EPR23563-120]
See all HHLA2 primary antibodies
Description

Rabbit monoclonal [EPR23563-120] to HHLA2
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Flow cyt: A-204 cells; HEK-293T cells transfected with Myc-His tagged HHLA2 overexpression vector.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
Flow Cyt	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23563-120
Isotype

IgG
Research areas

Flow Cytometry - Anti-HHLA2 antibody [EPR23563-120] (ab254367)

Flow cytometric analysis of HeLa (human cervix adenocarcinoma epithelial cell line, left) / A-204 (human muscle rhabdomyosarcoma, right) cells labelling HHLA2 with ab254367 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Negative control: Hela (PMID:23716685).

Gated on viable cells.
Flow Cytometry - Anti-HHLA2 antibody [EPR23563-120] (ab254367)

Flow cytometric analysis of 2% paraformaldehyde fixed, 0.1% Tween-20 permeabilized HEK-293T (Human embryonic kidney epithelial cell line) cells transfected with Myc-His tagged HHLA2 overexpression vector labelling HHLA2 with ab254367 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left).

A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Cells were surface stained with rabbit IgG or ab254367. Then fixed with 2% PFA for 10min followed by intracellularly stained with anti-myc tag conjugated to Alexa Fluor^®647.
Anti-HHLA2 antibody [EPR23563-120] (ab254367)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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