Anti-hCG beta antibody [5H4-E2] (ab9582)
![Anti-hCG beta antibody [5H4-E2] (ab9582)](https://www.abcam.com/ps/products/9/ab9582/Images/ab9582-294544-anti-hcg-beta-antibody-5h4-e2-immunohistochemistry.jpg "Anti-hCG beta antibody [5H4-E2] (ab9582)")
Key features and details
- Mouse monoclonal [5H4-E2] to hCG beta
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-hCG beta antibody [5H4-E2]
See all hCG beta primary antibodies -
Description
Mouse monoclonal [5H4-E2] to hCG beta -
Host species
Mouse -
Specificity
The antibody recognizes the whole hCG beta and the C-terminal end part of hCG beta, as well as the hCG dimer molecule. It does not react with hCG alpha, FSH, LH, TSH and albumins (evaluated by simple-binding ELISA using 1.00 µg/well purified antigens (WHO standards) and 1.00 µg/ml primary antibody concentrations). This antibody labels the cytoplasm of hCG beta producing (trophoblastic) cells in placenta, mola hydatidosa, choriocarcinoma, embryonal carcinoma and some undifferenciated lung cancers, as well as, the JAR choriocarcinoma cell line. It does not react with cells of normal endometrial, testicular tissues and lung (tested by immunohistochemistry on formalin-fixed, paraffin-embedded tissue sections using the Streptavidin-biotinylated peroxidase method). -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Does not react with: Horse -
Immunogen
Full length native protein (purified) corresponding to Human hCG beta.
Database link: P01233 -
Positive control
- ICC/IF: Human chorionic villus cells. IHC-P: Human fetal chorionic villus tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.097% Sodium azide
Constituents: Tris HCl, 0.2% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
5H4-E2 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hCG beta antibody [5H4-E2] (ab9582)
Formalin-fixed, paraffin-embedded human seminoma tissue stained for hCG beta with ab9582 at a 1/50 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hCG beta antibody [5H4-E2] (ab9582)](https://www.abcam.com/ps/products/9/ab9582/Images/ab9582-17296-anti-hcg-beta-antibody-5h4-e2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hCG beta antibody [5H4-E2] (ab9582) Image from Spitalieri P et al, Cloning Stem Cells. 2009 Dec;11(4):535-56, Fig 1, DOI: 10.1089=clo.2009.0046.ab9582 staining hCG beta in human fetal chorionic villus by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Samples were fixed in 4% paraformaldehyde and then incubated with ab9582 in PBT [3% bovine serum albumin (BSA) and 0.5% Triton X-100 in phosphate-buffered saline (PBS)] overnight at 4°C. After extensive washing with PBT, samples were incubated with a secondary antibody conjugated to Alexa-Fluor® 488 for 1 hour at room temperature in the dark. After nuclear staining with Hoechst slides were mounted with PBS/ glycerol.
