Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µg 307 566 ₸ 10 µg 102 522 ₸

Order now and get it on Wednesday March 10, 2021

Anti-HAUSP / USP7 antibody [3D8] (ab252877)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rat monoclonal [3D8] to HAUSP / USP7
Suitable for: IP, WB, ICC/IF
Reacts with: Human

Product name

Anti-HAUSP / USP7 antibody [3D8]
See all HAUSP / USP7 primary antibodies
Description

Rat monoclonal [3D8] to HAUSP / USP7
Host species

Rat

Tested Applications & Species

Application	Species
ICC/IF	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human HAUSP/ USP7. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q93009

Run BLAST with Run BLAST with
Positive control
- WB: HeLa, and MCF7 whole cell lysates. ICC/IF: HeLa and MCF7 cells. IP: HeLa and MCF7 whole cell lysates
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

3D8
Isotype

IgG2a
Light chain type

kappa
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-HAUSP / USP7 antibody [3D8] (ab252877)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling HAUSP / USP7 with ab252877 at 14.84 μg/mL, followed by ab150157 Goat Anti-rat IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HeLa cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150157 Goat Anti-rat IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.
Western blot - Anti-HAUSP / USP7 antibody [3D8] (ab252877)

All lanes : Anti-HAUSP / USP7 antibody [3D8] (ab252877) at 0.742 µg/ml

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rat IgG (H+L), HRP) (ab205720) at 1/5000 dilution

Predicted band size: 128 kDa
Observed band size: 128 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Exposure time: 7 seconds.
Immunoprecipitation - Anti-HAUSP / USP7 antibody [3D8] (ab252877)

HAUSP / USP7 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate with ab252877 at 24.733 μg/mL. Western blot was performed on the immunoprecipitate using ab252877 at 1/1000 dilution. Goat Anti-rat IgG (H+L), HRP) (ab205720) was used at 1/1000 dilution.

Lane 1: HeLa whole cell lysate 10 ug.

Lane 2: ab252877 IP in HeLa whole cell lysate.

Lane 3: Rat monoclonal IgG (ab18450) instead of ab252877 in HeLa whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 7 seconds.
Immunoprecipitation - Anti-HAUSP / USP7 antibody [3D8] (ab252877)

HAUSP / USP7 was immunoprecipitated from 0.35 mg MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate 10ug with ab252877 at 24.733 μg/mL. Western blot was performed on the immunoprecipitate using ab252877 at 1/1000 dilution. Goat Anti-rat IgG (H+L), HRP) (ab205720) was used at 1/1000 dilution.

Lane 1: MCF7 whole cell lysate 10ug.

Lane 2: ab252877 IP in MCF7 whole cell lysate.

Lane 3: Rat monoclonal IgG (ab18450) instead of ab252877 in MCF7 whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 7 seconds.
Immunocytochemistry/ Immunofluorescence - Anti-HAUSP / USP7 antibody [3D8] (ab252877)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MCF7 cells labelling HAUSP / USP7 with ab252877 at 14.84 μg/mL, followed by ab150157 Goat Anti-rat IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in MCF7 cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150157 Goat Anti-rat IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.
Anti-HAUSP / USP7 antibody [3D8] (ab252877)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com