Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HAUSP / USP7 antibody [3D8] (ab252877)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [3D8] to HAUSP / USP7
  • Suitable for: IP, WB, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-HAUSP / USP7 antibody [3D8]
    See all HAUSP / USP7 primary antibodies

  • Description

    Rat monoclonal [3D8] to HAUSP / USP7

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human HAUSP/ USP7. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q93009

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: HeLa, and MCF7 whole cell lysates. ICC/IF: HeLa and MCF7 cells. IP: HeLa and MCF7 whole cell lysates

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-HAUSP / USP7 antibody [3D8] (ab252877)
    Immunocytochemistry/ Immunofluorescence - Anti-HAUSP / USP7 antibody [3D8] (ab252877)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling HAUSP / USP7 with ab252877 at 14.84 μg/mL, followed by ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HeLa cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Western blot - Anti-HAUSP / USP7 antibody [3D8] (ab252877)
    Western blot - Anti-HAUSP / USP7 antibody [3D8] (ab252877)
    All lanes : Anti-HAUSP / USP7 antibody [3D8] (ab252877) at 0.742 µg/ml

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG (H+L), HRP) (ab205720) at 1/5000 dilution

    Predicted band size: 128 kDa
    Observed band size: 128 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 7 seconds.

  • Immunoprecipitation - Anti-HAUSP / USP7 antibody [3D8] (ab252877)
    Immunoprecipitation - Anti-HAUSP / USP7 antibody [3D8] (ab252877)

    HAUSP / USP7 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate with ab252877 at 24.733 μg/mL. Western blot was performed on the immunoprecipitate using ab252877 at 1/1000 dilution. Goat Anti-rat IgG (H+L), HRP) (ab205720) was used at 1/1000 dilution.

    Lane 1: HeLa whole cell lysate 10 ug.

    Lane 2: ab252877 IP in HeLa whole cell lysate.

    Lane 3: Rat monoclonal IgG (ab18450) instead of ab252877 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 7 seconds.

     

  • Immunoprecipitation - Anti-HAUSP / USP7 antibody [3D8] (ab252877)
    Immunoprecipitation - Anti-HAUSP / USP7 antibody [3D8] (ab252877)

    HAUSP / USP7 was immunoprecipitated from 0.35 mg MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate 10ug with ab252877 at 24.733 μg/mL. Western blot was performed on the immunoprecipitate using ab252877 at 1/1000 dilution. Goat Anti-rat IgG (H+L), HRP) (ab205720) was used at 1/1000 dilution.

    Lane 1: MCF7 whole cell lysate 10ug.

    Lane 2: ab252877 IP in MCF7 whole cell lysate.

    Lane 3: Rat monoclonal IgG (ab18450) instead of ab252877 in MCF7 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 7 seconds.

     

  • Immunocytochemistry/ Immunofluorescence - Anti-HAUSP / USP7 antibody [3D8] (ab252877)
    Immunocytochemistry/ Immunofluorescence - Anti-HAUSP / USP7 antibody [3D8] (ab252877)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MCF7 cells labelling HAUSP / USP7 with ab252877 at 14.84 μg/mL, followed by ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in MCF7 cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Anti-HAUSP / USP7 antibody [3D8] (ab252877)
    Anti-HAUSP / USP7 antibody [3D8] (ab252877)

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