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Anti-HAPLN1 antibody [EPR6338] (ab181997)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6338] to HAPLN1
  • Suitable for: Flow Cyt, WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-HAPLN1 antibody [EPR6338]
    See all HAPLN1 primary antibodies

  • Description

    Rabbit monoclonal [EPR6338] to HAPLN1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human spleen, MCF7 and Caco2 lysates; Human transitional cell carcinoma of bladder tissue; MCF7 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR6338

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-HAPLN1 antibody [EPR6338] (ab181997)
    Western blot - Anti-HAPLN1 antibody [EPR6338] (ab181997)
    All lanes : Anti-HAPLN1 antibody [EPR6338] (ab181997) at 1/1000 dilution

    Lane 1 : MCF7 lysate
    Lane 2 : Caco2 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L)-HRP at 1/1000 dilution

    Predicted band size: 40 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAPLN1 antibody [EPR6338] (ab181997)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HAPLN1 antibody [EPR6338] (ab181997)

    Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling HAPLN1 with ab181997 at 1/50 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-HAPLN1 antibody [EPR6338] (ab181997)
    Immunocytochemistry/ Immunofluorescence - Anti-HAPLN1 antibody [EPR6338] (ab181997)

    Immunofluorescent analysis of MCF7 cells (paraformaldehyde-fixed, 4%) labeling HAPLN1 with ab181997 at 1/250 dilution, followed by Goat anti rabbit IgG(Dylight 488) at 1/200 dilution and counter-stained with DAPI (blue).

  • Flow Cytometry - Anti-HAPLN1 antibody [EPR6338] (ab181997)
    Flow Cytometry - Anti-HAPLN1 antibody [EPR6338] (ab181997)

    Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling HAPLN1 with purified ab181997 at 1/2000 dilution (red). The secondary antibody was Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

  • Western blot - Anti-HAPLN1 antibody [EPR6338] (ab181997)
    Western blot - Anti-HAPLN1 antibody [EPR6338] (ab181997)
    Anti-HAPLN1 antibody [EPR6338] (ab181997) at 1/1000 dilution + Human spleen lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/1000 dilution

    Predicted band size: 40 kDa

  • Anti-HAPLN1 antibody [EPR6338] (ab181997)
    Anti-HAPLN1 antibody [EPR6338] (ab181997)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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