Anti-HACE1 antibody [EPR7962] - BSA and Azide free (ab248586)
![Anti-HACE1 antibody [EPR7962] - BSA and Azide free (ab248586)](https://www.abcam.com/ps/products/248/ab248586/Images/ab248586-410690-anti-hace1-antibody-epr7962-bsa-and-azide-free-western-blot.jpg "Anti-HACE1 antibody [EPR7962] - BSA and Azide free (ab248586)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7962] to HACE1 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-HACE1 antibody [EPR7962] - BSA and Azide free
See all HACE1 primary antibodies -
Description
Rabbit monoclonal [EPR7962] to HACE1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab248586 is the carrier-free version of ab133637. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab248586 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7962 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HACE1 antibody [EPR7962] - BSA and Azide free (ab248586)Anti-HACE1 antibody [EPR7962] (ab133637) at 1/5000 dilution (purified) + Rat brain tissue lysate at 20 µg
Secondary
Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 102 kDa
Observed band size: 102 kDaThis data was developed using ab133637, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-HACE1 antibody [EPR7962] - BSA and Azide free (ab248586)](https://www.abcam.com/ps/products/248/ab248586/Images/ab248586-407944-anti-hace1-antibody-epr7962-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-HACE1 antibody [EPR7962] - BSA and Azide free (ab248586)Anti-HACE1 antibody [EPR7962] (ab133637) at 1/5000 dilution (purified) + Mouse brain tissue lysate at 20 µg
Secondary
Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 102 kDa
Observed band size: 102 kDaThis data was developed using ab133637, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-HACE1 antibody [EPR7962] - BSA and Azide free (ab248586)](https://www.abcam.com/ps/products/248/ab248586/Images/ab248586-405998-anti-hace1-antibody-epr7962-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-HACE1 antibody [EPR7962] - BSA and Azide free (ab248586)All lanes : Anti-HACE1 antibody [EPR7962] (ab133637) at 1/1000 dilution (purified)
Lane 1 : HEK293 whole cell lysate
Lane 2 : SH-SY5Y whole cell lysate
Lane 3 : Human fetal brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 102 kDa
Observed band size: 102 kDaThis data was developed using ab133637, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-HACE1 antibody [EPR7962] - BSA and Azide free (ab248586)](https://www.abcam.com/ps/products/248/ab248586/Images/ab248586-402754-anti-hace1-antibody-epr7962-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-HACE1 antibody [EPR7962] - BSA and Azide free (ab248586)All lanes : Anti-HACE1 antibody [EPR7962] (ab133637) at 1/1000 dilution (unpurified)
Lane 1 : 293T cell lysate
Lane 2 : SH-SY5Y cell lysate at 10 µg
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 102 kDaThis data was developed using ab133637, the same antibody clone in a different buffer formulation.
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![Anti-HACE1 antibody [EPR7962] - BSA and Azide free (ab248586)](https://www.abcam.com/ps/products/248/ab248586/Images/ab248586-5-benefits-of-recombinant-antibodies.png)
Anti-HACE1 antibody [EPR7962] - BSA and Azide free (ab248586)