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Epigenetics and Nuclear Signaling DNA / RNA Translation tRNAs

Anti-GTF3C4 antibody (ab74229)

Anti-GTF3C4 antibody (ab74229)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to GTF3C4
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-GTF3C4 antibody
    See all GTF3C4 primary antibodies
  • Description

    Rabbit polyclonal to GTF3C4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1 and 50 of human GTF3C4 (NP_036336.1)

  • Positive control

    • HeLa whole cell lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antibody was affinity purified using an epitope specific to GTF3C4/TFIIIC90 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • tRNAs
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Polymerase associated factors
    • Pol III Transcription
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HAT

Images

  • Western blot - Anti-GTF3C4 antibody (ab74229)
    Western blot - Anti-GTF3C4 antibody (ab74229)
    All lanes : Anti-GTF3C4 antibody (ab74229) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique.

    Predicted band size: 92 kDa
    Observed band size: 95 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 185 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 10 seconds
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTF3C4 antibody (ab74229)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTF3C4 antibody (ab74229)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue labelling GTF3C4 with ab74229 at 1/200 (1µg/ml). Detection: DAB.
  • Immunoprecipitation - Anti-GTF3C4 antibody (ab74229)
    Immunoprecipitation - Anti-GTF3C4 antibody (ab74229)
    ab74229, at 1 µg/ml (used for IP at 3 µg/mg lysate)
    Lane 1: HeLa whole cell lysate at 1 mg for IP (20% of IP loaded).
    Lane 2: control IgG.
    Detection: Chemiluminescence with an exposure time of 3 seconds.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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