Anti-gtf3c3 antibody (ab74230)
Key features and details
- Rabbit polyclonal to gtf3c3
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-gtf3c3 antibody -
Description
Rabbit polyclonal to gtf3c3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
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Immunogen
Synthetic peptide corresponding to a region between residue 475 and 525 of human gtf3c3 (NP_036218.1)
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Positive control
- IP: HEK293T lysate; WB: HeLa, Jurkat cell lysates; IHC: human breast carcinoma
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-gtf3c3 antibody (ab74230)All lanes : Anti-gtf3c3 antibody (ab74230) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T cell at 50 µg
Lane 5 : mouse NIH3T3 cell at 50 µg
Predicted band size: 101 kDa
Observed band size: 101 kDa
Exposure time: 3 minutes
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gtf3c3 antibody (ab74230)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling gtf3c3 with ab74230 at 1/500 (0.4µg/ml). Detection: DAB. -
Immunoprecipitation - Anti-gtf3c3 antibody (ab74230)Lane 1: HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) with ab74230 used for IP at 3 µg/mg lysate.
Lane 2: control IgG.
Subsequent Western blot detection peformed using ab74230 at 1 µg/ml.
Detection: Chemiluminescence with an exposure time of 3 minutes.