Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] - BSA and Azide free (ab269859)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20890-48] to GSDMC2 + GSDMC3 - BSA and Azide free
  • Suitable for: IHC-Fr, WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse

Overview

  • Product name

    Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] - BSA and Azide free
    See all GSDMC2+GSDMC3 primary antibodies

  • Description

    Rabbit monoclonal [EPR20890-48] to GSDMC2 + GSDMC3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Fr, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    This product was produced with the following immunogens:
    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse stomach, small intestine, colon and caecum tissue lysates; HEK-293T transfected with mGSDMC2 expression vector, whole cell lysate; HEK-293T transfected with mGSDMC3 expression vector, whole cell lysate. IHC-P: Mouse stomach tissue. IHC-Fr: Mouse stomach and colon tissue.

  • General notes

    ab269859 is the carrier-free version of ab229896. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab269859 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20890-48

  • Isotype

    IgG

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSDMC2+GSDMC3 antibody [EPR20890-48] - BSA and Azide free (ab269859)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSDMC2+GSDMC3 antibody [EPR20890-48] - BSA and Azide free (ab269859)

    This data was developed using ab229896, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling GSDMC2+GSDMC3 with abab229896 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplamic staining on epithelial cells in mouse stomach (PMID: 17350798). The section was incubated with ab229896 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-GSDMC2+GSDMC3 antibody [EPR20890-48] - BSA and Azide free (ab269859)
    Western blot - Anti-GSDMC2+GSDMC3 antibody [EPR20890-48] - BSA and Azide free (ab269859)
    All lanes : Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896) at 1/1000 dilution

    Lane 1 : Wild-type mouse esophagus tissue lysate
    Lane 2 : mGSDMC1-4 knockout mouse esophagus tissue lysate
    Lane 3 : Wild-type mouse stomach tissue lysate
    Lane 4 : mGSDMC1-4 knockout mouse stomach tissue lysate
    Lane 5 : Wild-type mouse small intestine tissue lysate
    Lane 6 : mGSDMC1-4 knockout mouse small intestine tissue lysate
    Lane 7 : Wild-type mouse colon tissue lysate
    Lane 8 : mGSDMC1-4 knockout mouse colon tissue lysate
    Lane 9 : Wild-type mouse caecum tissue lysate
    Lane 10 : mGSDMC1-4 knockout mouse caecum tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 54 kDa
    Observed band size: 56 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab229896, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The image was kindly provided by Dr. Jiahuai Han (Xiamen University, China).

    Exposure time: 60 seconds.

  • Immunohistochemistry (Frozen sections) - Anti-GSDMC2+GSDMC3 antibody [EPR20890-48] - BSA and Azide free (ab269859)
    Immunohistochemistry (Frozen sections) - Anti-GSDMC2+GSDMC3 antibody [EPR20890-48] - BSA and Azide free (ab269859)

    This data was developed using ab229896, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse colon tissue labeling GSDMC2+GSDMC3 with ab229896 at 1/200 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on epithelial cells in mouse colon is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Western blot - Anti-GSDMC2+GSDMC3 antibody [EPR20890-48] - BSA and Azide free (ab269859)
    Western blot - Anti-GSDMC2+GSDMC3 antibody [EPR20890-48] - BSA and Azide free (ab269859)
    All lanes : Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896) at 1/1000 dilution

    Lane 1 : Mouse stomach tissue lysate
    Lane 2 : Mouse spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 54 kDa
    Observed band size: 54 kDa



    This data was developed using ab229896, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: spleen (PMID: 17350798).

    Exposure time: 54 seconds.

  • Immunohistochemistry (Frozen sections) - Anti-GSDMC2+GSDMC3 antibody [EPR20890-48] - BSA and Azide free (ab269859)
    Immunohistochemistry (Frozen sections) - Anti-GSDMC2+GSDMC3 antibody [EPR20890-48] - BSA and Azide free (ab269859)

    This data was developed using ab229896, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse stomach tissue labeling GSDMC2+GSDMC3 with ab229896 at 1/200 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on epithelial cells in mouse stomach is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Western blot - Anti-GSDMC2+GSDMC3 antibody [EPR20890-48] - BSA and Azide free (ab269859)
    Western blot - Anti-GSDMC2+GSDMC3 antibody [EPR20890-48] - BSA and Azide free (ab269859)
    All lanes : Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896) at 1/1000 dilution

    Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), whole cell lysate
    Lane 2 : HEK-293T transfected with mGSDMC1 expression vector, whole cell lysate
    Lane 3 : HEK-293T transfected with mGSDMC2 expression vector, whole cell lysate
    Lane 4 : HEK-293T transfected with mGSDMC3 expression vector, whole cell lysate
    Lane 5 : HEK-293T transfected with mGSDMC4 expression vector, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 54 kDa
    Observed band size: 56 kDa why is the actual band size different from the predicted?



    This data was developed using ab229896, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The image was kindly provided by Dr. Jiahuai Han (Xiamen University, China).

    Exposure time: 60 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSDMC2+GSDMC3 antibody [EPR20890-48] - BSA and Azide free (ab269859)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GSDMC2+GSDMC3 antibody [EPR20890-48] - BSA and Azide free (ab269859)

    This data was developed using ab229896, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling GSDMC2+GSDMC3 with abab229896 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining on mouse spleen (PMID: 17350798). The section was incubated with ab229896 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] - BSA and Azide free (ab269859)
    Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] - BSA and Azide free (ab269859)

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