Anti-GNB3 antibody [EPR8862] - BSA and Azide free (ab249165)
![Anti-GNB3 antibody [EPR8862] - BSA and Azide free (ab249165)](https://www.abcam.com/ps/products/249/ab249165/Images/ab249165-446348-anti-gnb3-antibody-epr8862-bsa-and-azide-free-western-blot.jpg "Anti-GNB3 antibody [EPR8862] - BSA and Azide free (ab249165)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8862] to GNB3 - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-GNB3 antibody [EPR8862] - BSA and Azide free
See all GNB3 primary antibodies -
Description
Rabbit monoclonal [EPR8862] to GNB3 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
Ab249165 is the carrier-free version of ab154866. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab249165 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8862 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-GNB3 antibody [EPR8862] - BSA and Azide free (ab249165)All lanes : Anti-GNB3 antibody [EPR8862] (ab154866) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Human fetal brain tissue lysate
Lane 3 : A673 cell lysate
Lane 4 : Human fetal lung tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 37 kDaThis data was developed using ab154866, the same antibody clone in a different buffer formulation.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNB3 antibody [EPR8862] - BSA and Azide free (ab249165)](https://www.abcam.com/ps/products/249/ab249165/Images/ab249165-7-anti-gnb3-antibody-epr8862-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNB3 antibody [EPR8862] - BSA and Azide free (ab249165)This data was developed using ab154866, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling GNB3 with ab154866 at 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNB3 antibody [EPR8862] - BSA and Azide free (ab249165)](https://www.abcam.com/ps/products/249/ab249165/Images/ab249165-5-anti-gnb3-antibody-epr8862-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNB3 antibody [EPR8862] - BSA and Azide free (ab249165)This data was developed using ab154866, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue using ab154866 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNB3 antibody [EPR8862] - BSA and Azide free (ab249165)](https://www.abcam.com/ps/products/249/ab249165/Images/ab249165-4-anti-gnb3-antibody-epr8862-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNB3 antibody [EPR8862] - BSA and Azide free (ab249165)This data was developed using ab154866, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human normal tonsil tissue using ab154866 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNB3 antibody [EPR8862] - BSA and Azide free (ab249165)](https://www.abcam.com/ps/products/249/ab249165/Images/ab249165-3-anti-gnb3-antibody-epr8862-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNB3 antibody [EPR8862] - BSA and Azide free (ab249165)This data was developed using ab154866, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human normal heart tissue using ab154866 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNB3 antibody [EPR8862] - BSA and Azide free (ab249165)](https://www.abcam.com/ps/products/249/ab249165/Images/ab249165-2-anti-gnb3-antibody-epr8862-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNB3 antibody [EPR8862] - BSA and Azide free (ab249165)This data was developed using ab154866, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154866 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNB3 antibody [EPR8862] - BSA and Azide free (ab249165)](https://www.abcam.com/ps/products/249/ab249165/Images/ab249165-1-anti-gnb3-antibody-epr8862-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNB3 antibody [EPR8862] - BSA and Azide free (ab249165)This data was developed using ab154866, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human retina tissue using ab154866 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Anti-GNB3 antibody [EPR8862] - BSA and Azide free (ab249165)](https://www.abcam.com/ps/products/249/ab249165/Images/ab249165-6-benefits-of-recombinant-antibodies.png)
Anti-GNB3 antibody [EPR8862] - BSA and Azide free (ab249165)