Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] - BSA and Azide free (ab275969)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19951-136] to Glutathione Peroxidase 3/GPx-3 - BSA and Azide free
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] - BSA and Azide free
    See all Glutathione Peroxidase 3/GPx-3 primary antibodies

  • Description

    Rabbit monoclonal [EPR19951-136] to Glutathione Peroxidase 3/GPx-3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
    Unsuitable for: Flow Cyt or ICC

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human kidney and lung tissue lysates; Human plasma. IHC-P: Human kidney and testis tissue. IP: Human kidney tissue lysate.

  • General notes

    ab275969 is the carrier-free version of ab275965. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    a275969 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] - BSA and Azide free (ab275969)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] - BSA and Azide free (ab275969)

    This data was developed using ab275965, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Glutathione Peroxidase 3/GPx-3 with ab275965 at 1/5000 (0.116 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on proximal tubules of human kidney. The section was incubated with ab275965 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] - BSA and Azide free (ab275969)
    Western blot - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] - BSA and Azide free (ab275969)
    All lanes : Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] (ab275965) at 1/1000 dilution

    Lane 1 : Human kidney tissue lysate
    Lane 2 : Human liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

    Predicted band size: 26 kDa
    Observed band size: 22,24 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab275965, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression pattern is consistent with what has been described in the literature (PMID: 20015939).

    Low expression: human liver (PMID: 9820194, 15294905).

    Exposure time: 92 seconds.

  • Immunoprecipitation - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] - BSA and Azide free (ab275969)
    Immunoprecipitation - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] - BSA and Azide free (ab275969)

    This data was developed using ab275965, the same antibody clone in a different buffer formulation.

    Glutathione Peroxidase 3/GPx-3 was immunoprecipitated from 0.35 mg Human kidney tissue lysate with ab275965 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275965 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Human kidney tissue lysate 10 ug

    Lane 2: ab275965 IP in Human kidney tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab275965 in human kidney tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 93 seconds.

     

  • Western blot - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] - BSA and Azide free (ab275969)
    Western blot - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] - BSA and Azide free (ab275969)
    All lanes : Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] (ab275965) at 1/1000 dilution

    Lane 1 : Human plasma
    Lane 2 : Human lung tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 26 kDa
    Observed band size: 24 kDa why is the actual band size different from the predicted?



    This data was developed using ab275965, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression pattern is consistent with what has been described in the literature (PMID: 20015939).

    Exposure time: Lane 1: 37 seconds Lane 2: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] - BSA and Azide free (ab275969)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] - BSA and Azide free (ab275969)

    This data was developed using ab275965, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Glutathione Peroxidase 3/GPx-3 with ab275965 at 1/5000 (0.116 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on basement membrane of human seminiferous tubules. The section was incubated with ab275965 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] - BSA and Azide free (ab275969)
    Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] - BSA and Azide free (ab275969)

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