Anti-Glutaminase antibody (ab131554)
Key features and details
- Rabbit polyclonal to Glutaminase
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Glutaminase antibody
See all Glutaminase primary antibodies -
Description
Rabbit polyclonal to Glutaminase -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Chimpanzee, Macaque monkey, Gorilla
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Immunogen
Synthetic peptide corresponding to Human Glutaminase aa 600 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab156175) -
Positive control
- This antibody gave a positive signal in both Mouse Cortex and Mouse Brain. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.01% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab131554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes ICC/IF Use a concentration of 1 µg/ml. WB Use a concentration of 1 µg/ml. Detects a band of approximately 71 kDa (predicted molecular weight: 73 kDa). Target
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Function
Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. -
Tissue specificity
KGA is expressed predominantly in brain and kidney but not in liver, isoform 3 is expressed principally in cardiac muscle and pancreas but not in liver or brain, and isoform 2 is expressed solely in cardiac and skeletal muscle. -
Sequence similarities
Belongs to the glutaminase family.
Contains 1 ANK repeat. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 2744 Human
- Entrez Gene: 14660 Mouse
- Entrez Gene: 24398 Rat
- Omim: 138280 Human
- SwissProt: O94925 Human
- SwissProt: D3Z7P3 Mouse
- SwissProt: P13264 Rat
- Unigene: 116448 Human
see all -
Alternative names
- AAD20 antibody
- DKFZp686O15119 antibody
- FLJ10358 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Glutaminase antibody (ab131554)
ICC/IF image of ab131554 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab131554 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-Glutaminase antibody (ab131554)All lanes : Anti-Glutaminase antibody (ab131554) at 1 µg/ml
Lane 1 : Mouse Cortex Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 73 kDa
Observed band size: 71 kDa why is the actual band size different from the predicted?
Additional bands at: 39 kDa, 92 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 seconds
The band observed at 71 kDa could potentially be a cleaved form of Glutaminase due to the presence of a 16 amino acid transit peptide. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab131554 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Protocols
Datasheets and documents
References (1)
ab131554 has been referenced in 1 publication.
- Moreno-Felici J et al. Phosphoenolpyruvate from Glycolysis and PEPCK Regulate Cancer Cell Fate by Altering Cytosolic Ca2. Cells 9:N/A (2019). PubMed: 31861674
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Glutaminase antibody (ab131554)
ICC/IF image of ab131554 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab131554 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-Glutaminase antibody (ab131554)All lanes : Anti-Glutaminase antibody (ab131554) at 1 µg/ml
Lane 1 : Mouse Cortex Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 73 kDa
Observed band size: 71 kDa why is the actual band size different from the predicted?
Additional bands at: 39 kDa, 92 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 seconds
The band observed at 71 kDa could potentially be a cleaved form of Glutaminase due to the presence of a 16 amino acid transit peptide. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab131554 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
