Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GluN2D antibody (ab35448)

Key features and details

  • Rabbit polyclonal to GluN2D
  • Suitable for: WB
  • Reacts with: Mouse, Rat
  • Isotype: IgG

Overview

  • Product name

    Anti-GluN2D antibody
    See all GluN2D primary antibodies

  • Description

    Rabbit polyclonal to GluN2D

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human GluN2D aa 1300 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    Database link: O15399
    (Peptide available as ab35447)

  • Positive control

    • This antibody gave a positive signal in Mouse Brain Tissue Lysate (day 0 and day 7) and Rat Brain Tissue Lysate (day 0 and day 7)

  • General notes

     This product was previously labelled as Glutamate receptor ionotropic

     

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab35448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Mouse
Rat
All applications
Human
Application Abreviews Notes
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 143 kDa (predicted molecular weight: 143 kDa).
Notes
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 143 kDa (predicted molecular weight: 143 kDa).

Target

  • Function

    NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.

  • Sequence similarities

    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2D/GRIN2D subfamily.

  • Cellular localization

    Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • EB11 antibody
    • estrogen receptor binding CpG island antibody
    • GluN2D antibody
    • Glutamate [NMDA] receptor subunit epsilon 4 antibody
    • Glutamate [NMDA] receptor subunit epsilon-4 antibody
    • Glutamate receptor ionotropic antibody
    • glutamate receptor ionotropic NMDA 2D antibody
    • Glutamate receptor, ionotropic, N-methyl D-aspartate 2D antibody
    • Grin2d antibody
    • N methyl D aspartate receptor subtype 2D antibody
    • N methyl d aspartate receptor subunit 2D antibody
    • N-methyl D-aspartate receptor subtype 2D antibody
    • NMDA 2D antibody
    • NMDAR2D antibody
    • NMDE4 antibody
    • NMDE4_HUMAN antibody
    • NR2D antibody
    see all

Images

  • Western blot - Anti-GluN2D antibody (ab35448)
    Western blot - Anti-GluN2D antibody (ab35448)
    All lanes : Anti-GluN2D antibody (ab35448) at 1 µg/ml

    Lane 1 : Mouse brain tissue lysate - total protein (0 days) (ab7188)
    Lane 2 : Brain (Mouse) Tissue Lysate, 7 days old
    Lane 3 : Brain (Rat) Tissue Lysate, 0 Days old
    Lane 4 : Brain (Rat) Tissue Lysate, 7 days old

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 143 kDa
    Observed band size: 143 kDa
    Additional bands at: ~200 kDa, 125 kDa, 45 kDa, 60 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes


    Ab35448 detects NR2D at the predicted MW of 143 kDa consistent with published data on NR2D protein (Dunah et al, 1996; 1998); we are unsure as to the identity of the lower bands.

References (1)

Publishing research using ab35448? Please let us know so that we can cite the reference in this datasheet.

ab35448 has been referenced in 1 publication.

  • Zhou X  et al. Reduced NMDAR1 expression in the Sp4 hypomorphic mouse may contribute to endophenotypes of human psychiatric disorders. Hum Mol Genet 19:3797-805 (2010). WB ; Mouse . PubMed: 20634195

Images

  • Western blot - Anti-GluN2D antibody (ab35448)
    Western blot - Anti-GluN2D antibody (ab35448)
    All lanes : Anti-GluN2D antibody (ab35448) at 1 µg/ml

    Lane 1 : Mouse brain tissue lysate - total protein (0 days) (ab7188)
    Lane 2 : Brain (Mouse) Tissue Lysate, 7 days old
    Lane 3 : Brain (Rat) Tissue Lysate, 0 Days old
    Lane 4 : Brain (Rat) Tissue Lysate, 7 days old

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 143 kDa
    Observed band size: 143 kDa
    Additional bands at: ~200 kDa, 125 kDa, 45 kDa, 60 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes


    Ab35448 detects NR2D at the predicted MW of 143 kDa consistent with published data on NR2D protein (Dunah et al, 1996; 1998); we are unsure as to the identity of the lower bands.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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