Anti-Glucose Transporter GLUT3 antibody (ab41525)
Key features and details
- Rabbit polyclonal to Glucose Transporter GLUT3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Glucose Transporter GLUT3 antibody
See all Glucose Transporter GLUT3 primary antibodies -
Description
Rabbit polyclonal to Glucose Transporter GLUT3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Mouse Glucose Transporter GLUT3 (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P32037 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Antibody stabilization buffer. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antigen-sepharose affinity column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Glucose Transporter GLUT3 antibody (ab41525)All lanes : Anti-Glucose Transporter GLUT3 antibody (ab41525) at 1/2000 dilution
Lanes 1 & 4 & 7 & 10 : Sal 3 month cortical extract
Lanes 2 & 5 & 8 & 11 : Glu 3 month cortical extract
Lanes 3 & 9 & 12 : CB 3 month cortical extract
Lane 6 : GB 3 month cortical extract
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Alkaline phosphatase-conjugated goat anti-rabbit IgG at 1/5000 dilution10% SDS-PAGE gel.
Incubated with the primary antibody for 2 hours at room temperature followed by 3x10 minutes washes in TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT3 antibody (ab41525)ab41525 staining GLUT3 in human duodenum.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
