Anti-GLP1 antibody [8G9] (ab26278)
![Anti-GLP1 antibody [8G9] (ab26278)](https://www.abcam.com/ps/products/26/ab26278/Images/ab26278-170652-anti-glp1-amidated-antibody-8g9-immunohistochemistry.jpg "Anti-GLP1 antibody [8G9] (ab26278)")
Key features and details
- Mouse monoclonal [8G9] to GLP-1
- Suitable for: IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
-
Product name
Anti-GLP-1 antibody [8G9]
See all GLP-1 primary antibodies -
Description
Mouse monoclonal [8G9] to GLP-1 -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: a wide range of other species
-
Immunogen
Synthetic peptide corresponding to Human GLP-1 aa 1-100. ab26278 reacts with the amidated C-Terminus of GLP-1(7-36) coupled to a carrier.
-
Epitope
C-terminal epitope of GLP-1(7-36)amide -
Positive control
- IHC-P/IHC-Fr: rat colon, rat and mouse pancreas tissue.
-
General notes
ab26278 should be used as capture with biotinylated version ab121057 as detection.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 2.9% Sodium chloride -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
8G9 -
Myeloma
x63-Ag8.653 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP1 antibody [8G9] (ab26278)
ab26278 (1µg/ml) staining GLP1 in Human pancreas, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP1 antibody [8G9] (ab26278)](https://www.abcam.com/ps/products/26/ab26278/Images/ab26278-343892-anti-glp1-antibody-immunofluorescence-pancreas-mice.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP1 antibody [8G9] (ab26278)Immunofluorescence analysis of diabetic mice pancreas tissue stained for GLP1 using ab26278 at 1/500 dilution.
