Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GIPC3 antibody [EPR15315] (ab186426)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15315] to GIPC3
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-GIPC3 antibody [EPR15315]
    See all GIPC3 primary antibodies

  • Description

    Rabbit monoclonal [EPR15315] to GIPC3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • K562, HeLa and Human lymph node lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15315

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-GIPC3 antibody [EPR15315] (ab186426)
    Western blot - Anti-GIPC3 antibody [EPR15315] (ab186426)
    All lanes : Anti-GIPC3 antibody [EPR15315] (ab186426) at 1/10000 dilution

    Lane 1 : K562 lysate
    Lane 2 : Human lymph node lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/10000 dilution

    Predicted band size: 34 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-GIPC3 antibody [EPR15315] (ab186426)
    Western blot - Anti-GIPC3 antibody [EPR15315] (ab186426)
    Anti-GIPC3 antibody [EPR15315] (ab186426) at 1/50000 dilution + HeLa cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?

  • Immunoprecipitation - Anti-GIPC3 antibody [EPR15315] (ab186426)
    Immunoprecipitation - Anti-GIPC3 antibody [EPR15315] (ab186426)

    Western blot analysis of GIPC3 in HeLa cell lysate immunoprecipitated using ab186426 at 1/50 dilution (Lane 1). Lane 2: PBS instead of HeLa lysate. Anti-Rabbit IgG (HRP) specific to the non-reduced form of IgG was used as a secondary antibody at 1/1500 dilution.

  • Anti-GIPC3 antibody [EPR15315] (ab186426)
    Anti-GIPC3 antibody [EPR15315] (ab186426)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Clear all

Recently viewed products

© 2021 Astana Biomed Group LLP. All rights reserved.