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Epigenetics and Nuclear Signaling DNA / RNA Translation Mito. Translation

Anti-GFM1 antibody [EPR12263] - BSA and Azide free (ab249664)

Anti-GFM1 antibody [EPR12263] - BSA and Azide free (ab249664)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12263] to GFM1 - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-GFM1 antibody [EPR12263] - BSA and Azide free
    See all GFM1 primary antibodies
  • Description

    Rabbit monoclonal [EPR12263] to GFM1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Human GFM1. The exact sequence is proprietary.
    Database link: Q96RP9

  • General notes

    ab249664 is the carrier-free version of ab171945.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12263
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Mito. Translation
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial translation
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Nucleotide metabolism

Images

  • Western blot - Anti-GFM1 antibody [EPR12263] - BSA and Azide free (ab249664)
    Western blot - Anti-GFM1 antibody [EPR12263] - BSA and Azide free (ab249664)
    All lanes : Anti-GFM1 antibody [EPR12263] (ab171945) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : NCI 460 cell line lysate
    Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
    Lane 4 : 293T (Human embryonic kidney epithelial cell) cell lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 83 kDa



    This data was developed using ab171945, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFM1 antibody [EPR12263] - BSA and Azide free (ab249664)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFM1 antibody [EPR12263] - BSA and Azide free (ab249664)
    This data was developed using ab171945, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling GFM1 with ab171945 at 1/50 Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFM1 antibody [EPR12263] - BSA and Azide free (ab249664)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFM1 antibody [EPR12263] - BSA and Azide free (ab249664)
    This data was developed using ab171945, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue labeling GFM1 with ab171945 at 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-GFM1 antibody [EPR12263] - BSA and Azide free (ab249664)
    Anti-GFM1 antibody [EPR12263] - BSA and Azide free (ab249664)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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