Anti-GCC185 antibody (ab128173)
Key features and details
- Rabbit polyclonal to GCC185
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GCC185 antibody -
Description
Rabbit polyclonal to GCC185 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human GCC185 aa 1-50. (NP_852118.1).
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Positive control
- HeLa, 293T and Jurkat whole cell lysate (ab7899)
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General notes
Protein previously labeled as GCC2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab128173 is affinity purified using an epitope specific to GCC185 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GCC185 antibody (ab128173)All lanes : Anti-GCC185 antibody (ab128173) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 196 kDa
Exposure time: 30 seconds
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Immunoprecipitation - Anti-GCC185 antibody (ab128173)Detection of GCC185 by Western Blot of Immunprecipitate.
ab128173, at 1 µg/ml, staining GCC185 in HeLa whole cell lysate immunoprecipitated using ab128173 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.