Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GBP2 antibody [EPR13206] - N-terminal (ab179829)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13206] to GBP2 - N-terminal
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-GBP2 antibody [EPR13206] - N-terminal
    See all GBP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR13206] to GBP2 - N-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human GBP2 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: P32456

  • Positive control

    • WB: Human spleen, IM-9, HACAT, A549 and K562 lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR13206

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-GBP2 antibody [EPR13206] - N-terminal (ab179829)
    Western blot - Anti-GBP2 antibody [EPR13206] - N-terminal (ab179829)
    All lanes : Anti-GBP2 antibody [EPR13206] - N-terminal (ab179829) at 1/1000 dilution

    Lane 1 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate
    Lane 2 : GBP2 knockout A549 (Human lung carcinoma cell line) whole cell lysate
    Lane 3 : K562 (Human chronic myelogenous leukemia lymphoblast cell line) whole cell lysate
    Lane 4 : HaCaT (Human keratinocyte cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 67 kDa
    Observed band size: 67 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab179829 observed at 67 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab179829 Anti-GBP2 antibody [EPR13206] - N-terminal was shown to specifically react with GBP2 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267218 (knockout cell lysate ab257962) was used. Wild-type and GBP2 knockout samples were subjected to SDS-PAGE. ab179829 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-GBP2 antibody [EPR13206] - N-terminal (ab179829)
    Western blot - Anti-GBP2 antibody [EPR13206] - N-terminal (ab179829)
    All lanes : Anti-GBP2 antibody [EPR13206] - N-terminal (ab179829) at 1/1000 dilution

    Lane 1 : Wild-type A549 cell lysate
    Lane 2 : GBP2 knockout A549 cell lysate
    Lane 3 : K-562 cell lysate
    Lane 4 : HaCaT cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 67 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?



    Lanes 1-4: Merged signal (red and green). Green - ab179829 observed at 70 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab179829 Anti-GBP2 antibody [EPR13206] - N-terminal was shown to specifically react with GBP2 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267219 (knockout cell lysate ab257963) was used. Wild-type and GBP2 knockout samples were subjected to SDS-PAGE. ab179829 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-GBP2 antibody [EPR13206] - N-terminal (ab179829)
    Western blot - Anti-GBP2 antibody [EPR13206] - N-terminal (ab179829)
    All lanes : Anti-GBP2 antibody [EPR13206] - N-terminal (ab179829) at 1/1000 dilution

    Lane 1 : Human spleen cell lysate
    Lane 2 : IM-9 cell lysate
    Lane 3 : HACAT cell lysate
    Lane 4 : K562 cell lysate

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 67 kDa

  • Anti-GBP2 antibody [EPR13206] - N-terminal (ab179829)
    Anti-GBP2 antibody [EPR13206] - N-terminal (ab179829)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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