Anti-GBP2 antibody [EPR13206] - N-terminal (ab179829)
![Anti-GBP2 antibody [EPR13206] - N-terminal (ab179829)](https://www.abcam.com/ps/products/179/ab179829/Images/ab179829-412656-anti-gbp2-antibody-epr13206-n-terminal-western-blot-human-knockout-lysate.jpg "Anti-GBP2 antibody [EPR13206] - N-terminal (ab179829)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13206] to GBP2 - N-terminal
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-GBP2 antibody [EPR13206] - N-terminal
See all GBP2 primary antibodies -
Description
Rabbit monoclonal [EPR13206] to GBP2 - N-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human GBP2 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P32456 -
Positive control
- WB: Human spleen, IM-9, HACAT, A549 and K562 lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13206 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GBP2 antibody [EPR13206] - N-terminal (ab179829)All lanes : Anti-GBP2 antibody [EPR13206] - N-terminal (ab179829) at 1/1000 dilution
Lane 1 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate
Lane 2 : GBP2 knockout A549 (Human lung carcinoma cell line) whole cell lysate
Lane 3 : K562 (Human chronic myelogenous leukemia lymphoblast cell line) whole cell lysate
Lane 4 : HaCaT (Human keratinocyte cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 67 kDa
Observed band size: 67 kDaLanes 1-4: Merged signal (red and green). Green - ab179829 observed at 67 kDa. Red - loading control ab8245 observed at 36 kDa.
ab179829 Anti-GBP2 antibody [EPR13206] - N-terminal was shown to specifically react with GBP2 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267218 (knockout cell lysate ab257962) was used. Wild-type and GBP2 knockout samples were subjected to SDS-PAGE. ab179829 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-GBP2 antibody [EPR13206] - N-terminal (ab179829)](https://www.abcam.com/ps/products/179/ab179829/Images/ab179829-410145-anti-gbp2-antibody-epr13206-n-terminal-western-blot-human-knockout-lysate.jpg)
Western blot - Anti-GBP2 antibody [EPR13206] - N-terminal (ab179829)All lanes : Anti-GBP2 antibody [EPR13206] - N-terminal (ab179829) at 1/1000 dilution
Lane 1 : Wild-type A549 cell lysate
Lane 2 : GBP2 knockout A549 cell lysate
Lane 3 : K-562 cell lysate
Lane 4 : HaCaT cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 67 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab179829 observed at 70 kDa. Red - loading control ab8245 observed at 36 kDa.
ab179829 Anti-GBP2 antibody [EPR13206] - N-terminal was shown to specifically react with GBP2 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267219 (knockout cell lysate ab257963) was used. Wild-type and GBP2 knockout samples were subjected to SDS-PAGE. ab179829 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-GBP2 antibody [EPR13206] - N-terminal (ab179829)](https://www.abcam.com/ps/products/179/ab179829/Images/ab179829-208093-anti-gbp2-antibody-epr13206-n-terminal-western-blot.jpg)
Western blot - Anti-GBP2 antibody [EPR13206] - N-terminal (ab179829)All lanes : Anti-GBP2 antibody [EPR13206] - N-terminal (ab179829) at 1/1000 dilution
Lane 1 : Human spleen cell lysate
Lane 2 : IM-9 cell lysate
Lane 3 : HACAT cell lysate
Lane 4 : K562 cell lysate
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 67 kDa
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![Anti-GBP2 antibody [EPR13206] - N-terminal (ab179829)](https://www.abcam.com/ps/products/179/ab179829/Images/ab179829-4-benefits-of-recombinant-antibodies.png)
Anti-GBP2 antibody [EPR13206] - N-terminal (ab179829)
