Anti-GATA4 antibody (ab227512)
Key features and details
- Rabbit polyclonal to GATA4
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-GATA4 antibody
See all GATA4 primary antibodies -
Description
Rabbit polyclonal to GATA4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Pig
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Immunogen
Recombinant fragment within Human GATA4 (internal sequence). The exact sequence is proprietary.
Database link: P43694 -
Positive control
- WB: HeLa whole cell extract; NIH/3T3, JC, BCL-1, HeLa and A549 whole cell lysates; Rat heart lysate. ICC/IF: HeLa cells. IHC-P: Mouse lung tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-GATA4 antibody (ab227512)
Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for GATA4 (green) using ab227512 at 1/500 dilution in ICC/IF.
Counterstain: Hoechst 33342 (blue).
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Western blot - Anti-GATA4 antibody (ab227512)All lanes : Anti-GATA4 antibody (ab227512) at 1/3000 dilution
Lane 1 : Non-transfected HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : GATA4 shRNA transfected HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 45 kDa10% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA4 antibody (ab227512)Paraffin-embedded mouse lung tissue stained for GATA4 with ab227512 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-GATA4 antibody (ab227512)Anti-GATA4 antibody (ab227512) at 1/1000 dilution + Rat heart lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 45 kDa10% SDS-PAGE gel.
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Western blot - Anti-GATA4 antibody (ab227512)All lanes : Anti-GATA4 antibody (ab227512) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 45 kDa10% SDS PAGE gel.
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Western blot - Anti-GATA4 antibody (ab227512)All lanes : Anti-GATA4 antibody (ab227512) at 1/1000 dilution
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 2 : JC whole cell lysate
Lane 3 : BCL-1 whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 45 kDa10% SDS-PAGE gel.