Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Wednesday March 10, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR7157] to GARS
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-GARS antibody [EPR7157]
See all GARS primary antibodies
Description

Rabbit monoclonal [EPR7157] to GARS
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human GARS aa 700-800 (C terminal). The exact sequence is proprietary.
Positive control
- Jurkat, HeLa, U87-MG, 293T, C6, RAW 264.7, and Neuro-2a cell lysates; Human colon tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR7157
Isotype

IgG
Research areas

Western blot - Anti-GARS antibody [EPR7157] (ab125008)

All lanes : Anti-GARS antibody [EPR7157] (ab125008) at 1/1000 dilution

Lane 1 : Jurkat cell lysate
Lane 2 : Hela cell lysate
Lane 3 : U87-MG cell lysate
Lane 4 : 293T cell lysate
Lane 5 : C6 cell lysate
Lane 6 : RAW 264.7 cell lysate
Lane 7 : Neuro-2a cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 83 kDa
Observed band size: 75 kDa
why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GARS antibody [EPR7157] (ab125008)

ab125008 at 1/50 staining GARS in paraffin embedded Human colon tissue by immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-GARS antibody [EPR7157] (ab125008)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com