Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GARS antibody [EPR7157] (ab125008)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7157] to GARS
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GARS antibody [EPR7157]
    See all GARS primary antibodies

  • Description

    Rabbit monoclonal [EPR7157] to GARS

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human GARS aa 700-800 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Jurkat, HeLa, U87-MG, 293T, C6, RAW 264.7, and Neuro-2a cell lysates; Human colon tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR7157

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-GARS antibody [EPR7157] (ab125008)
    Western blot - Anti-GARS antibody [EPR7157] (ab125008)
    All lanes : Anti-GARS antibody [EPR7157] (ab125008) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : Hela cell lysate
    Lane 3 : U87-MG cell lysate
    Lane 4 : 293T cell lysate
    Lane 5 : C6 cell lysate
    Lane 6 : RAW 264.7 cell lysate
    Lane 7 : Neuro-2a cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 83 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GARS antibody [EPR7157] (ab125008)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GARS antibody [EPR7157] (ab125008)

    ab125008 at 1/50 staining GARS in paraffin embedded Human colon tissue by immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-GARS antibody [EPR7157] (ab125008)
    Anti-GARS antibody [EPR7157] (ab125008)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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