Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GAPDH antibody [FF26A] (ab59164)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [FF26A] to GAPDH
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-GAPDH antibody [FF26A]
    See all GAPDH primary antibodies

  • Description

    Mouse monoclonal [FF26A] to GAPDH

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • Positive control

    • IP: HepG2 and MDA-MB-231 whole cell lysate. WB: Human brain, heart, kidney and spleen tissue lysate. HeLa, HepG2, Jurkat, A549 and MDA-MB-231 whole cell lysate.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-GAPDH antibody [FF26A] (ab59164)
    Western blot - Anti-GAPDH antibody [FF26A] (ab59164)
    All lanes : Anti-GAPDH antibody [FF26A] (ab59164) at 0.441 µg/ml

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg
    Lane 2 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg
    Lane 3 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg
    Lane 4 : A549 (human lung carcinoma epithelial cell), whole cell lysate at 20 µg
    Lane 5 : MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg
    Lane 6 : Human brain tissue lysate at 10 µg
    Lane 7 : Human heart tissue lysate at 10 µg
    Lane 8 : Human kidney tissue lysate at 10 µg
    Lane 9 : Human spleen tissue lysate at 10 µg

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 36 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-5: 7 secs; Lanes 6-9: 10 secs.

  • Immunoprecipitation - Anti-GAPDH antibody [FF26A] (ab59164)
    Immunoprecipitation - Anti-GAPDH antibody [FF26A] (ab59164)

    GADPH was immunoprecipitated from HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with ab59164 at 14/7µg/ml. Western blot was performed from the immunoprecipitate using ab59164 at 1/1000 dilution. Anti-mouse IgG for IP (HRP) (ab131368) was used at 1/5000 dilution.
    Lane 1: HepG2 whole cell lysate 10µg (Input).
    Lane 2: ab59164 IP in HepG2 whole cell lysate.
    Lane 3: Mouse monoclonal IgG instead of ab59164 in HepG2 whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 5 secs.

  • Immunoprecipitation - Anti-GAPDH antibody [FF26A] (ab59164)
    Immunoprecipitation - Anti-GAPDH antibody [FF26A] (ab59164)

    GADPH was immunoprecipitated from MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate with ab59164 at 14.7µg/ml. Western blot was performed from the immunoprecipitate using ab59164 at 1/1000 dilution. Anti-mouse IgG for IP (HRP) (ab131368) was used at 1/5000 dilution.
    Lane 1: MDA-MB-231 whole cell lysate 10µg (Input).
    Lane 2: ab59164 IP in MDA-MB-231 whole cell lysate.
    Lane 3: Mouse monoclonal IgG instead of ab59164 in MDA-MB-231 whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 5 secs.

  • Anti-GAPDH antibody [FF26A] (ab59164)
    Anti-GAPDH antibody [FF26A] (ab59164)

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