Anti-GAP43 antibody [5E8] (ab277627)
![Anti-GAP43 antibody [5E8] (ab277627)](https://www.abcam.com/ps/products/277/ab277627/Images/ab277627-438733-AntiGAP43-antibody-5E8-ICC-Embryonic-ratE20-cortical-neuronglial-cells.JPG "Anti-GAP43 antibody [5E8] (ab277627)")
Key features and details
- Mouse monoclonal [5E8] to GAP43
- Suitable for: WB, ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-GAP43 antibody [5E8]
See all GAP43 primary antibodies -
Description
Mouse monoclonal [5E8] to GAP43 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein corresponding to Human GAP43.
Database link: P17677 -
Positive control
- WB: Rat brain and spinal cord, mouse brain and spinal cord, SH-SY5Y cells. ICC: Embryonic rat (E20) cortical neuron-glial cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.03% Sodium azide
Constituents: 49.07% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from Tissue Culture supernatant. -
Clonality
Monoclonal -
Clone number
5E8 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry - Anti-GAP43 antibody [5E8] (ab277627)
Cortical neuron-glial cell culture from E20 rat stained for GAP43 (red) using ab277627 at 1/1000 dilution and counterstained with chicken pAb to GFAP (green) at 1/2000 dilution in ICC. The blue DAPI is staining nuclear DNA.
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![Western blot - Anti-GAP43 antibody [5E8] (ab277627)](https://www.abcam.com/ps/products/277/ab277627/Images/ab277627-438732-AntiGAP43-antibody-5E8-WB-rat-brain-rat-spinal-cord-mouse-brain-mouse-spinal-cord-and-SHSY5Y-cells.JPG)
Western blot - Anti-GAP43 antibody [5E8] (ab277627)Lanes 2-7 : Anti-GAP43 antibody [5E8] (ab277627) at 1/2000 dilution
Lane 1 : Molecular weight ladder
Lane 2 : Rat brain
Lane 3 : Rat spinal cord
Lane 4 : Mouse brain
Lane 5 : Mouse spinal cord
Lane 6 : C6 cells
Lane 7 : SH-SY5Y cells
Predicted band size: 24 kDa
