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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Proteasome

Anti-Gankyrin antibody [EPR14456(2)] - BSA and Azide free (ab250947)

Anti-Gankyrin antibody [EPR14456(2)] - BSA and Azide free (ab250947)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14456(2)] to Gankyrin - BSA and Azide free
  • Suitable for: Flow Cyt, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Gankyrin antibody [EPR14456(2)] - BSA and Azide free
    See all Gankyrin primary antibodies
  • Description

    Rabbit monoclonal [EPR14456(2)] to Gankyrin - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Gankyrin aa 100-200. The exact sequence is proprietary.
    Database link: O75832

  • Positive control

    • Hela, HepG2, 293, C6, Raw264.7 and K562 cell lysates. HeLa cells.
  • General notes

    ab250947 is the carrier-free version of ab188315. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250947 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    EPR14456(2)
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Proteasome
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other

Images

  • Western blot - Anti-Gankyrin antibody [EPR14456(2)] - BSA and Azide free (ab250947)
    Western blot - Anti-Gankyrin antibody [EPR14456(2)] - BSA and Azide free (ab250947)
    All lanes : Anti-Gankyrin antibody [EPR14456(2)] (ab188315) at 1/1000 dilution

    Lane 1 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : PSMD10 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : K562 (Human chronic myelogenous leukemia lymphoblast cell line) whole cell lysate
    Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 24 kDa
    Observed band size: 24 kDa



    This data was developed using ab188315, the same antibody clone in a different buffer formulation.

    Lanes 1-4: Merged signal (red and green). Green - ab188315 observed at 24 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab188315 Anti-Gankyrin antibody [EPR14456(2)] was shown to specifically react with Gankyrin in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265680 (knockout cell lysate ab258146) was used. Wild-type and Gankyrin knockout samples were subjected to SDS-PAGE. ab188315 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-Gankyrin antibody [EPR14456(2)] - BSA and Azide free (ab250947)
    Flow Cytometry - Anti-Gankyrin antibody [EPR14456(2)] - BSA and Azide free (ab250947)
    This data was developed using ab188315, the same antibody clone in a different buffer formulation.Flow cytometric analysis of HeLa cells fixed in 2% paraformaldehyde labeling Gankyrin with ab188315 at 1/110 dilution and Goat anti-rabbit IgG (FITC) at 1/150 dilution. Rabbit monoclonal IgG was used as an isotype control.
  • Western blot - Anti-Gankyrin antibody [EPR14456(2)] - BSA and Azide free (ab250947)
    Western blot - Anti-Gankyrin antibody [EPR14456(2)] - BSA and Azide free (ab250947)
    All lanes : Anti-Gankyrin antibody [EPR14456(2)] (ab188315) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : Raw 264.7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 24 kDa



    This data was developed using ab188315, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-Gankyrin antibody [EPR14456(2)] - BSA and Azide free (ab250947)
    Western blot - Anti-Gankyrin antibody [EPR14456(2)] - BSA and Azide free (ab250947)
    All lanes : Anti-Gankyrin antibody [EPR14456(2)] (ab188315) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 24 kDa



    This data was developed using ab188315, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-Gankyrin antibody [EPR14456(2)] - BSA and Azide free (ab250947)
    Western blot - Anti-Gankyrin antibody [EPR14456(2)] - BSA and Azide free (ab250947)
    All lanes : Anti-Gankyrin antibody [EPR14456(2)] (ab188315) at 1/10000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : Hela cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 24 kDa



    This data was developed using ab188315, the same antibody clone in a different buffer formulation.

     

  • Anti-Gankyrin antibody [EPR14456(2)] - BSA and Azide free (ab250947)
    Anti-Gankyrin antibody [EPR14456(2)] - BSA and Azide free (ab250947)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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