Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23383-89] to Galectin 13/PLAC8 + Galectin-16
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89]

  • Description

    Rabbit monoclonal [EPR23383-89] to Galectin 13/PLAC8 + Galectin-16

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human placenta tissue lysate; His-tagged human Galectin 13/PLAC8 recombinant protein; His-lif tagged human Galectin 16 recombinant protein (aa 1-141). IHC-P: Human placenta tissue. IP: Human placenta tissue lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23383-89

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716)
    Western blot - Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716)
    All lanes : Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716) at 1/1000 dilution

    Lane 1 : Human placenta tissue lysate
    Lane 2 : Human skeletal muscle tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

    Predicted band size: 16 kDa
    Observed band size: 15 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID: 10527825, 19497882).

    Negative control: Human skeletal muscle (PMID: 10527825).

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 3 minutes.

  • Western blot - Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716)
    Western blot - Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716)
    All lanes : Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716) at 1/1000 dilution

    Lane 1 : His-tagged human Galectin 13/PLAC8 recombinant protein (aa 1-139, ab124309), 20 ng
    Lane 2 : His-lif tagged human Galectin 16 recombinant protein (aa 1-141) 5 µL

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 16 kDa
    Observed band size: 15/55 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This product has cross reaction with Galectin 16.

    Sample loaded onto lane 2 was extract of HEK293 expressing Galectin 16, which contains a lif tag (40 kDa). 

    Exposure time: 70 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling Galectin 13+Galectin 16 with ab272716 at 1/2000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human placenta (PMID: 25191322). The section was incubated with ab272716 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunoprecipitation - Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716)
    Immunoprecipitation - Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716)

    Galectin 13+Galectin 16 was immunoprecipitated from 0.35 mg human placenta tissue lysate with ab272716 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab272716 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/1000 dilution.

    Lane 1: Human placenta tissue lysate 10µg

    Lane 2: ab272716 IP in human placenta tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab272716 in human placenta tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

     

  • Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716)
    Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] (ab272716)

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