Anti-G9P antibody (ab100996)
Key features and details
- Rabbit polyclonal to G9P
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-G9P antibody
See all G9P primary antibodies -
Description
Rabbit polyclonal to G9P -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human G9P aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab115936) -
Positive control
- This antibody gave a positive signal in Human spleen tissue lysate as well as the following whole cell lysates: MDA MB 231; MDA MB 361; HeLa.
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General notes
Previously labelled as KIR2DL4.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab100996 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications ChimpanzeeMacaque monkeyGorillaOrangutanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 41 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 41 kDa).Target
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Function
Receptor on natural killer (NK) cells for HLA-C alleles. Inhibits the activity of NK cells thus preventing cell lysis. -
Sequence similarities
Belongs to the immunoglobulin superfamily.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 3805 Human
- Omim: 604945 Human
- SwissProt: Q99706 Human
- Unigene: 651287 Human
- Unigene: 661219 Human
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Alternative names
- CD_antigen=CD158d antibody
- CD158 antigen like family member D antibody
- CD158 antigen-like family member D antibody
see all
Images
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Western blot - Anti-G9P antibody (ab100996)All lanes : Anti-G9P antibody (ab100996) at 1 µg/ml
Lane 1 : Human spleen tissue lysate - total protein (ab29699)
Lane 2 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutesThe band observed at 39 kDa could potentially be a cleaved form of G9P due to the presence of a 21 amino acid signal peptide.
Datasheets and documents
References (0)
ab100996 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-G9P antibody (ab100996)All lanes : Anti-G9P antibody (ab100996) at 1 µg/ml
Lane 1 : Human spleen tissue lysate - total protein (ab29699)
Lane 2 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutesThe band observed at 39 kDa could potentially be a cleaved form of G9P due to the presence of a 21 amino acid signal peptide.
