Anti-FXYD6/Plp antibody [EPR9004(2)] (ab181254)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9004(2)] to FXYD6/Plp
- Suitable for: IP, WB
- Reacts with: Human
Overview
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Product name
Anti-FXYD6/Plp antibody [EPR9004(2)]
See all FXYD6/Plp primary antibodies -
Description
Rabbit monoclonal [EPR9004(2)] to FXYD6/Plp -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human -
Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human testis and Human fetal brain lysates.
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General notes
This product was previously labelled as FXYD6
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9004(2) -
Isotype
IgG
Images
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Anti-FXYD6/Plp antibody [EPR9004(2)] (ab181254) at 1/50000 dilution + Human fetal brain lysate at 20 µg
Secondary
goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 11 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
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Anti-FXYD6/Plp antibody [EPR9004(2)] (ab181254) at 1/10000 dilution + Human testis lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Developed using the ECL technique.
Predicted band size: 11 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
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Western blot analysis on immunoprecipitation from Human testis lysate labeling FXYD6/Plp using ab181254 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1000 dilution.
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