Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Furin antibody [EPR14674] (ab183495)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14674] to Furin
Suitable for: ICC/IF, IHC-P, WB
Reacts with: Human

Product name

Anti-Furin antibody [EPR14674]
See all Furin primary antibodies
Description

Rabbit monoclonal [EPR14674] to Furin
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- HepG2, Hela, Caco-2 and U87-MG lysates, Human gastric carcinoma, Human prostate tissues, Hela and HepG2 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 20% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14674
Isotype

IgG
Research areas

Western blot - Anti-Furin antibody [EPR14674] (ab183495)

All lanes : Anti-Furin antibody [EPR14674] (ab183495) at 1/5000 dilution

Lane 1 : HepG2 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : U87-MG whole cell lysate
Lane 4 : Caco-2 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 87 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Furin antibody [EPR14674] (ab183495)

Immunocytochemical analysis of HepG2 cells fixed in 4% paraformaldehyde labeling ab183495 at 1/250 dilution and Goat anti rabbit IgG(Alexa Fluor® 555) at 1/200. Counterstained with DAPI.
Immunocytochemistry/ Immunofluorescence - Anti-Furin antibody [EPR14674] (ab183495)

Immunocytochemical analysis of HeLa cells fixed in 20 °C acetone labeling Furin with ab183495 at 1/250 dilution and Goat anti rabbit IgG(Alexa Fluor® 555) at 1/200 dilution. Counterstained with DAPI.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Furin antibody [EPR14674] (ab183495)

Immunohistochemical analysis of paraffin-embedded Human prostate labeling Furin with ab183495 at 1/50 dilution and counterstained with hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Furin antibody [EPR14674] (ab183495)

Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling Furin with ab183495 at 1/50 dilution and counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-Furin antibody [EPR14674] (ab183495)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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