Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Furin antibody [EPR14674] (ab183495)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14674] to Furin
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Furin antibody [EPR14674]
    See all Furin primary antibodies

  • Description

    Rabbit monoclonal [EPR14674] to Furin

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2, Hela, Caco-2 and U87-MG lysates, Human gastric carcinoma, Human prostate tissues, Hela and HepG2 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 20% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR14674

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Furin antibody [EPR14674] (ab183495)
    Western blot - Anti-Furin antibody [EPR14674] (ab183495)
    All lanes : Anti-Furin antibody [EPR14674] (ab183495) at 1/5000 dilution

    Lane 1 : HepG2 whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : U87-MG whole cell lysate
    Lane 4 : Caco-2 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 87 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Furin antibody [EPR14674] (ab183495)
    Immunocytochemistry/ Immunofluorescence - Anti-Furin antibody [EPR14674] (ab183495)

    Immunocytochemical analysis of HepG2 cells fixed in 4% paraformaldehyde labeling ab183495 at 1/250 dilution and Goat anti rabbit IgG(Alexa Fluor® 555) at 1/200.  Counterstained with DAPI.

  • Immunocytochemistry/ Immunofluorescence - Anti-Furin antibody [EPR14674] (ab183495)
    Immunocytochemistry/ Immunofluorescence - Anti-Furin antibody [EPR14674] (ab183495)

    Immunocytochemical analysis of HeLa cells fixed in 20 °C acetone labeling Furin with ab183495 at 1/250 dilution and Goat anti rabbit IgG(Alexa Fluor® 555) at 1/200 dilution.  Counterstained with DAPI.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Furin antibody [EPR14674] (ab183495)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Furin antibody [EPR14674] (ab183495)

    Immunohistochemical analysis of paraffin-embedded Human prostate labeling Furin with ab183495 at 1/50 dilution and counterstained with hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Furin antibody [EPR14674] (ab183495)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Furin antibody [EPR14674] (ab183495)

    Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling Furin with ab183495 at 1/50 dilution and counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Furin antibody [EPR14674] (ab183495)
    Anti-Furin antibody [EPR14674] (ab183495)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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