Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FRZB antibody [EPR23904-20] (ab273582)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23904-20] to FRZB
  • Suitable for: Flow Cyt, IP, ICC/IF, WB
  • Reacts with: Mouse

Overview

  • Product name

    Anti-FRZB antibody [EPR23904-20]
    See all FRZB primary antibodies

  • Description

    Rabbit monoclonal [EPR23904-20] to FRZB

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Mouse
    IP
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse brain and testis tissue lysates. ICC/IF: Neuro-2a cells. Flow Cyt: Neuro-2a cells. IP: Mouse testis tissue lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-FRZB antibody [EPR23904-20] (ab273582)
    Western blot - Anti-FRZB antibody [EPR23904-20] (ab273582)
    All lanes : Anti-FRZB antibody [EPR23904-20] (ab273582) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse testis tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 36 kDa
    Observed band size: 36 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 28300015).

    Exposure time: 3 minutes.

  • Immunoprecipitation - Anti-FRZB antibody [EPR23904-20] (ab273582)
    Immunoprecipitation - Anti-FRZB antibody [EPR23904-20] (ab273582)

    FRZB was immunoprecipitated from 0.35 mg Mouse testis tissue lysate with ab273582 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab273582 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse testis tissue lysate 10 ug

    Lane 2: ab273582 IP in Mouse testis tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab273582 in mouse testis tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes

     

  • Flow Cytometry - Anti-FRZB antibody [EPR23904-20] (ab273582)
    Flow Cytometry - Anti-FRZB antibody [EPR23904-20] (ab273582)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized Neuro-2a (Mouse neuroblastoma neuroblast) cells labelling FRZB with ab273582 at 1/50 dilution (1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-FRZB antibody [EPR23904-20] (ab273582)
    Immunocytochemistry/ Immunofluorescence - Anti-FRZB antibody [EPR23904-20] (ab273582)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Neuro-2a cells labelling FRZB with ab273582 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in Neuro-2a cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Anti-FRZB antibody [EPR23904-20] (ab273582)
    Anti-FRZB antibody [EPR23904-20] (ab273582)

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