Anti-FRAS1 antibody (ab240583)
Key features and details
- Rabbit polyclonal to FRAS1
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-FRAS1 antibody
See all FRAS1 primary antibodies -
Description
Rabbit polyclonal to FRAS1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
-
Immunogen
Synthetic peptide within Human FRAS1 aa 3025-3075. The exact sequence is proprietary.
Database link: Q86XX4 -
Positive control
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab240583 was affinity purified using an epitope specific to FRAS1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-FRAS1 antibody (ab240583)All lanes : Anti-FRAS1 antibody (ab240583) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 443 kDa
Exposure time: 3 minutesLysates prepared using NETN lysis buffer.
-
Immunoprecipitation - Anti-FRAS1 antibody (ab240583)FRAS1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer. Western blot was performed from the immunoprecipitate using ab240583 at 1/100 dilution.
Lane 1: ab240583 used for IP at 20 µl per reaction.
Lane 2: Control IgG.Detection: Chemiluminescence with an exposure time of 30 seconds.
