Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FOXP3 antibody [221D] (ab253297)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [221D] to FOXP3
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-FOXP3 antibody [221D]
    See all FOXP3 primary antibodies

  • Description

    Mouse monoclonal [221D] to FOXP3

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T transfected with FOXP3 (WT) expression vector containing a GFP-myc tag, whole cell lysate. IHC-P: Human stomach carcinoma, tonsil and colon tissue; Mouse spleen and thymus tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-FOXP3 antibody [221D] (ab253297)
    Western blot - Anti-FOXP3 antibody [221D] (ab253297)
    All lanes : Anti-FOXP3 antibody [221D] (ab253297) at 1.514 µg/ml

    Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a GFP-myc tag, whole cell lysate
    Lane 2 : HEK-293T transfected with FOXP3 (WT) expression vector containing a GFP-myc tag, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 47 kDa
    Observed band size: 73 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 seconds


    The molecular weight observed was in consistant with the literature (PMID: 17586580).

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [221D] (ab253297)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [221D] (ab253297)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling FOXP3 with ab253297 at 1/1000 dilution (1.514 µg/ml) followed by a ready to use secondary. Nuclear staining on the human tonsil. The section was incubated with ab253297 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use secondary.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [221D] (ab253297)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [221D] (ab253297)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling FOXP3 with ab253297 at 1/1000 dilution (1.514 µg/ml) followed by a ready to use secondary. Nuclear staining on the stroma cells in the human colon. The section was incubated with ab253297 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use secondary.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [221D] (ab253297)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [221D] (ab253297)

    Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue labeling FOXP3 with ab253297 at 1/1000 dilution (1.514 µg/ml) followed by a ready to use secondary. Nuclear staining on the stroma cells in the human stomach carcinoma. The section was incubated with ab253297 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use secondary.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [221D] (ab253297)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [221D] (ab253297)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling FOXP3 with ab253297 at 1/1000 dilution (1.514 µg/ml) followed by a ready to use secondary. Nuclear staining on mouse spleen tissue. The section was incubated with ab253297 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use secondary.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [221D] (ab253297)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [221D] (ab253297)

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling FOXP3 with ab253297 at 1/1000 dilution (1.514 µg/ml) followed by a ready to use secondary. Nuclear staining on mouse thymus tissue. The section was incubated with ab253297 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use secondary.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-FOXP3 antibody [221D] (ab253297)
    Anti-FOXP3 antibody [221D] (ab253297)

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