Anti-FOXB2 antibody [EPR11476] (ab170897)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11476] to FOXB2
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-FOXB2 antibody [EPR11476]
See all FOXB2 primary antibodies -
Description
Rabbit monoclonal [EPR11476] to FOXB2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human FOXB2 aa 400 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q5VYV0 -
Positive control
- HeLa and LoVo cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11476 -
Isotype
IgG -
Research areas
Images
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Western blot analysis on immunoprecipitation pellet from HeLa cell lysate (Lane 1) or 1X PBS (negative control) (Lane 2) using ab170897 and HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.
Lane 1 Primary: ab170897, 1/1000 dilution
Sample: Immunoprecipitation pellet from HeLa cell lysate
Secondary: HRP-conjugated anti-rabbit IgGLane 2 Primary: ab170897, 1/1000 dilution
Sample: 1X PBS (negative control)
Secondary: HRP-conjugated anti-rabbit IgG -
All lanes : Anti-FOXB2 antibody [EPR11476] (ab170897) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : LoVo cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 45 kDa
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