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Anti-FHL2 antibody (ab12327)

Key features and details

  • Rabbit polyclonal to FHL2
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-FHL2 antibody
    See all FHL2 primary antibodies

  • Description

    Rabbit polyclonal to FHL2

  • Host species

    Rabbit

  • Specificity

    Reactivity with mouse FHL2 is unlikely owing to divergence of this ortholog from the peptide used for antibody production. The epitope recognized by ab1237 maps to a region between residues 175 and 225 of human Four and a Half LIM domains 2 using the numbering given in entry NP_963849.1 (GeneID 2274).

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human FHL2 aa 175-225.
    Database link: Q14192
    (Peptide available as ab119360)

  • Positive control

    • WB: U-2 OS and HeLa cell lysates; HeLa nuclear extract.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7
    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    This antibody is affinity purified.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-FHL2 antibody (ab12327)
    Western blot - Anti-FHL2 antibody (ab12327)
    All lanes : Anti-FHL2 antibody (ab12327) at 1/1000 dilution

    Lane 1 : Wild-type U-2 OS cell lysate
    Lane 2 : FHL2 knockout U-2 OS cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 40 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 32 kDa
    Observed band size: 32 kDa



    Lanes 1 - 3: Merged signal (red and green). Green - ab12327 observed at 32 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.

    ab12327 was shown to react with FHL2 in wild-type U-2 OS cells in western blot with loss of signal observed in FHL2 knockout sample. Wild-type and FHL2 knockout U-2 OS cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab12327 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-FHL2 antibody (ab12327)
    Western blot - Anti-FHL2 antibody (ab12327)

    Samples:

    Nuclear extract (NE) from HeLa cells. Antibody: Affinity purified rabbit anti-FHL2 antibody ab12327 used at 0.3 µg/ml. Detection: Chemiluminescence with an exposure time of 5 minutes. Samples: Nuclear extract (NE) from HeLa cells. Antibody: Affinity purified rabbit anti-FHL2 antibody ab12327 used at 0.3 µg/ml. Detection: Chemiluminescence with an exposure time of 5 minutes.

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