Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FGL1 antibody [EPR24018-27] (ab275091)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24018-27] to FGL1
  • Suitable for: IHC-P, IP, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-FGL1 antibody [EPR24018-27]
    See all FGL1 primary antibodies

  • Description

    Rabbit monoclonal [EPR24018-27] to FGL1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human liver tissue lysate: HepG2 and Huh7 whole cell lysates. IHC-P: Human liver and hepatocellular carcinoma tissue. Flow cyt: HepG2 cells. IP: HepG2 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR24018-27

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGL1 antibody [EPR24018-27] (ab275091)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGL1 antibody [EPR24018-27] (ab275091)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling FGL1 with ab275091 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in human liver (PMID: 11470158). The section was incubated with ab275091 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-FGL1 antibody [EPR24018-27] (ab275091)
    Western blot - Anti-FGL1 antibody [EPR24018-27] (ab275091)
    All lanes : Anti-FGL1 antibody [EPR24018-27] (ab275091) at 1/1000 dilution

    Lane 1 : Human liver tissue lysate
    Lane 2 : Human brain tissue lysate
    Lane 3 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

    Predicted band size: 36 kDa
    Observed band size: 34 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 15 seconds.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 11004478).

    Negative control: Human brain (PMID: 11470158).

     

  • Flow Cytometry - Anti-FGL1 antibody [EPR24018-27] (ab275091)
    Flow Cytometry - Anti-FGL1 antibody [EPR24018-27] (ab275091)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cells labelling FGL1 with ab275091 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-FGL1 antibody [EPR24018-27] (ab275091)
    Immunoprecipitation - Anti-FGL1 antibody [EPR24018-27] (ab275091)

    FGL1 was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with ab275091 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275091 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate 10 ug

    Lane 2: ab275091 IP in HepG2 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of abID1 in HepG2 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGL1 antibody [EPR24018-27] (ab275091)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGL1 antibody [EPR24018-27] (ab275091)

    Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling FGL1 with ab275091 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in human hepatocellular carcinoma (PMID: 14981537). The section was incubated with ab275091 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-FGL1 antibody [EPR24018-27] (ab275091)
    Western blot - Anti-FGL1 antibody [EPR24018-27] (ab275091)
    Anti-FGL1 antibody [EPR24018-27] (ab275091) at 1/1000 dilution + Huh7 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 36 kDa
    Observed band size: 34 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGL1 antibody [EPR24018-27] (ab275091)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGL1 antibody [EPR24018-27] (ab275091)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling FGL1 with ab275091 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining in human cerebrum (PMID: 14981537). The section was incubated with ab275091 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-FGL1 antibody [EPR24018-27] (ab275091)
    Anti-FGL1 antibody [EPR24018-27] (ab275091)

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