Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FGF2 antibody [EPR20145-227] - BSA and Azide free (ab251551)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20145-227] to FGF2 - BSA and Azide free
  • Suitable for: IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-FGF2 antibody [EPR20145-227] - BSA and Azide free
    See all FGF2 primary antibodies

  • Description

    Rabbit monoclonal [EPR20145-227] to FGF2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251551 is the carrier-free version of ab215373. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251551 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-FGF2 antibody [EPR20145-227] - BSA and Azide free (ab251551)
    Western blot - Anti-FGF2 antibody [EPR20145-227] - BSA and Azide free (ab251551)
    All lanes : Anti-FGF2 antibody [EPR20145-227] (ab215373) at 1/1000 dilution

    Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 2 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
    Lane 3 : SK-OV-3 (Human ovarian cancer cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 30 kDa
    Observed band size: 18,22,24,34 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab215373, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: Lanes 1 and 2: 3 minutes; Lane 3: 1 minute.

    The MW observed is consistent with the literatures (PMID:18850066, PMID: 24827991).

  • Western blot - Anti-FGF2 antibody [EPR20145-227] - BSA and Azide free (ab251551)
    Western blot - Anti-FGF2 antibody [EPR20145-227] - BSA and Azide free (ab251551)
    Anti-FGF2 antibody [EPR20145-227] (ab215373) at 1/10000 dilution + Recombinant human FGF2 active protein (aa143-288) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 30 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?


    Exposure time: 3 seconds


    This data was developed using ab215373, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-FGF2 antibody [EPR20145-227] - BSA and Azide free (ab251551)
    Western blot - Anti-FGF2 antibody [EPR20145-227] - BSA and Azide free (ab251551)
    All lanes : Anti-FGF2 antibody [EPR20145-227] (ab215373) at 1/1000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : Human fetal brain lysate
    Lane 3 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 30 kDa
    Observed band size: 24 kDa why is the actual band size different from the predicted?



    This data was developed using ab215373, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: Lanes 1 and 2: 3 minutes; Lane 3: 1 minute.

    The MW observed is consistent with the literatures (PMID:18850066, PMID: 24827991).

  • Immunoprecipitation - Anti-FGF2 antibody [EPR20145-227] - BSA and Azide free (ab251551)
    Immunoprecipitation - Anti-FGF2 antibody [EPR20145-227] - BSA and Azide free (ab251551)
    This data was developed using ab215373, the same antibody clone in a different buffer formulation.FGF2 was immunoprecipitated from 0.35 mg of K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate with ab215373 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab215373 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1,000 dilution Lane 1: K562 whole cell lysate 10µg (Input). Lane 2: ab215373 IP in K562 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab215373 in K562 whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 1 second.
  • Anti-FGF2 antibody [EPR20145-227] - BSA and Azide free (ab251551)
    Anti-FGF2 antibody [EPR20145-227] - BSA and Azide free (ab251551)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.