Anti-Factor D/CFD antibody [EPR17004-240] - BSA and Azide free (ab251541)
![Anti-Factor D/CFD antibody [EPR17004-240] - BSA and Azide free (ab251541)](https://www.abcam.com/ps/products/251/ab251541/Images/ab251541-403427-anti-factor-d-cfd-antibody-epr17004-240-bsa-and-azide-free-western-blot.jpg "Anti-Factor D/CFD antibody [EPR17004-240] - BSA and Azide free (ab251541)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17004-240] to Factor D/CFD - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse
Overview
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Product name
Anti-Factor D/CFD antibody [EPR17004-240] - BSA and Azide free
See all Factor D/CFD primary antibodies -
Description
Rabbit monoclonal [EPR17004-240] to Factor D/CFD - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody detects a distinct epitope compared to ab181307.
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Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab251541 is the carrier-free version of ab213177 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab251541 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as Factor D
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR17004-240 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Factor D/CFD antibody [EPR17004-240] - BSA and Azide free (ab251541)Anti-Factor D/CFD antibody [EPR17004-240] (ab213177) at 1/5000 dilution + His-tagged Mouse Factor D/CFD (aa26-259) recombinant protein at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 28 kDa
Observed band size: 29 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsThis data was developed using ab213177, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Factor D/CFD antibody [EPR17004-240] - BSA and Azide free (ab251541)](https://www.abcam.com/ps/products/251/ab251541/Images/ab251541-399398-anti-factor-d-cfd-antibody-epr17004-240-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Factor D/CFD antibody [EPR17004-240] - BSA and Azide free (ab251541)All lanes : Anti-Factor D/CFD antibody [EPR17004-240] (ab213177) at 1/1000 dilution
Lane 1 : Mouse plasma
Lane 2 : Mouse serum
Lane 3 : Mouse lung lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 28 kDa
Observed band size: 45-50 kDa why is the actual band size different from the predicted?This data was developed using ab213177, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: Lanes 1-2: 1 second; Lane 3: 15 seconds.
The higher molecular mass corresponds to the glycosylated protein. The expression profile observed is consistent with what has been described in the literature (PMID: 20038603; 12949072).
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![Anti-Factor D/CFD antibody [EPR17004-240] - BSA and Azide free (ab251541)](https://www.abcam.com/ps/products/251/ab251541/Images/ab251541-3-benefits-of-recombinant-antibodies.png)
Anti-Factor D/CFD antibody [EPR17004-240] - BSA and Azide free (ab251541)