Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-EXOC2 antibody [EPR9420] (ab140620)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9420] to EXOC2
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-EXOC2 antibody [EPR9420]
    See all EXOC2 primary antibodies

  • Description

    Rabbit monoclonal [EPR9420] to EXOC2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human EXOC2 (N terminal). The exact sequence is proprietary.

  • Positive control

    • WB: HeLa, SH-SY5Y , Daudi, BxPC-3 cell lysates; Human placenta and Fetal brain lysates. Flow Cyt: BxPC-3 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR9420

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-EXOC2 antibody [EPR9420] (ab140620)
    Western blot - Anti-EXOC2 antibody [EPR9420] (ab140620)
    All lanes : Anti-EXOC2 antibody [EPR9420] (ab140620) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : EXOC2 knockout HeLa cell lysate
    Lane 3 : SH-SY5Y cell lysate
    Lane 4 : Daudi cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 104 kDa
    Observed band size: 104 kDa



    Lanes 1- 4: Merged signal (red and green). Green - ab140620 observed at 104 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab140620 was shown to react with EXOC2 in wild-type HeLa cells in western blot. The band observed in knockout cell line ab265839 (knockout cell lysate ab257944) lane below 104kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HeLa and EXOC2 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab140620 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-EXOC2 antibody [EPR9420] (ab140620)
    Western blot - Anti-EXOC2 antibody [EPR9420] (ab140620)
    All lanes : Anti-EXOC2 antibody [EPR9420] (ab140620) at 1/1000 dilution

    Lane 1 : BxPC-3 cell lysate
    Lane 2 : Human placenta tissue lysate
    Lane 3 : Fetal brain tissue lysate
    Lane 4 : SH-SY5Y cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 104 kDa

  • Flow Cytometry - Anti-EXOC2 antibody [EPR9420] (ab140620)
    Flow Cytometry - Anti-EXOC2 antibody [EPR9420] (ab140620)
    Flow cytometric analysis of permeabilized BxPC-3 cells labelling EXOC2 with ab140620 at 1/10 dilution (red) or a Rabbit IgG negative control (green).
  • Anti-EXOC2 antibody [EPR9420] (ab140620)
    Anti-EXOC2 antibody [EPR9420] (ab140620)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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