Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-EWSR1/EWS antibody [21B1] - BSA and Azide free (ab255785)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [21B1] to EWSR1/EWS - BSA and Azide free
  • Suitable for: IP, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-EWSR1/EWS antibody [21B1] - BSA and Azide free
    See all EWSR1/EWS primary antibodies

  • Description

    Rat monoclonal [21B1] to EWSR1/EWS - BSA and Azide free

  • Host species

    Rat

  • Tested applications

    Suitable for: IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: K562, HeLa, Ramos and F9 whole cell lysate. IP: HeLa and K562 whole cell lysate.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab255785 is the carrier-free version of ab252829. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunoprecipitation - Anti-EWSR1/EWS antibody [21B1] - BSA and Azide free (ab255785)
    Immunoprecipitation - Anti-EWSR1/EWS antibody [21B1] - BSA and Azide free (ab255785)

    EWSR1/EWS was immunoprecipitated from K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate with ab252829 at 12.03µg/ml (2μg in 0.35mg lysates). Western blot was performed from the immunoprecipitate using ab252829 at 1/1000 dilution. Goat Anti-Rat IgG (H+L), HRP) (ab205720) was used at 1/5000 dilution.
    Lane 1: K562 whole cell lysate 10µg (Input).
    Lane 2: ab252829 IP in K562 whole cell lysate.
    Lane 3: Rat monoclonal IgG instead of ab252829 in K562 whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 secs.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252829).

  • Immunoprecipitation - Anti-EWSR1/EWS antibody [21B1] - BSA and Azide free (ab255785)
    Immunoprecipitation - Anti-EWSR1/EWS antibody [21B1] - BSA and Azide free (ab255785)

    EWSR1/EWS was immunoprecipitated from HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab252829 at 12.03µg/ml (2μg in 0.35mg lysates). Western blot was performed from the immunoprecipitate using ab252829 at 1/1000 dilution. Goat Anti-Rat IgG (H+L), HRP) (ab205720) was used at 1/5000 dilution.
    Lane 1: HeLa whole cell lysate 10µg (Input).
    Lane 2: ab252829 IP in HeLa whole cell lysate.
    Lane 3: Rat monoclonal IgG instead of ab252829 in HeLa whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 secs.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252829).

  • Western blot - Anti-EWSR1/EWS antibody [21B1] - BSA and Azide free (ab255785)
    Western blot - Anti-EWSR1/EWS antibody [21B1] - BSA and Azide free (ab255785)
    All lanes : Anti-EWSR1/EWS antibody [21B1] (ab252829) at 0.361 µg/ml

    Lane 1 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
    Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 3 : Ramos (human Burkitt's lymphoma B lymphocyte), whole cell lysate
    Lane 4 : F9 (mouse embryonal carcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

    Predicted band size: 68 kDa


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 15 secs.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252829).

  • Anti-EWSR1/EWS antibody [21B1] - BSA and Azide free (ab255785)
    Anti-EWSR1/EWS antibody [21B1] - BSA and Azide free (ab255785)

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