Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-EVPL antibody [EPR14688] (ab185217)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14688] to EVPL
  • Suitable for: IHC-P, WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-EVPL antibody [EPR14688]
    See all EVPL primary antibodies

  • Description

    Rabbit monoclonal [EPR14688] to EVPL

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal skin and A431 cell lysates. Human skin tissue. HeLa cells.

  • General notes

     

     

    Previously labelled as Envoplakin. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-EVPL antibody [EPR14688] (ab185217)
    Western blot - Anti-EVPL antibody [EPR14688] (ab185217)
    Anti-EVPL antibody [EPR14688] (ab185217) at 1/1000 dilution + Human fetal skin lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 232 kDa

  • Western blot - Anti-EVPL antibody [EPR14688] (ab185217)
    Western blot - Anti-EVPL antibody [EPR14688] (ab185217)
    Anti-EVPL antibody [EPR14688] (ab185217) at 1/10000 dilution + A431 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 232 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EVPL antibody [EPR14688] (ab185217)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EVPL antibody [EPR14688] (ab185217)

    Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling EVPL with ab185217 at 1/1000 dilution. The slide is counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-EVPL antibody [EPR14688] (ab185217)
    Flow Cytometry - Anti-EVPL antibody [EPR14688] (ab185217)

    Flow Cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling EVPL with ab185217 at 1/110 dilution (red) compared with a rabbit monoclonal IgG control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

  • Anti-EVPL antibody [EPR14688] (ab185217)
    Anti-EVPL antibody [EPR14688] (ab185217)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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