Anti-EVPL antibody [EPR14688] (ab185217)
![Anti-EVPL antibody [EPR14688] (ab185217)](https://www.abcam.com/ps/products/185/ab185217/Images/ab185217-218426-anti-evpl-antibody-epr14688-western-blot.jpg "Anti-EVPL antibody [EPR14688] (ab185217)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14688] to EVPL
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-EVPL antibody [EPR14688]
See all EVPL primary antibodies -
Description
Rabbit monoclonal [EPR14688] to EVPL -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal skin and A431 cell lysates. Human skin tissue. HeLa cells.
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General notes
Previously labelled as Envoplakin.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14688 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-EVPL antibody [EPR14688] (ab185217)Anti-EVPL antibody [EPR14688] (ab185217) at 1/1000 dilution + Human fetal skin lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 232 kDa
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![Western blot - Anti-EVPL antibody [EPR14688] (ab185217)](https://www.abcam.com/ps/products/185/ab185217/Images/ab185217-218429-anti-evpl-antibody-epr14688-western-blot.jpg)
Western blot - Anti-EVPL antibody [EPR14688] (ab185217)Anti-EVPL antibody [EPR14688] (ab185217) at 1/10000 dilution + A431 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 232 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EVPL antibody [EPR14688] (ab185217)](https://www.abcam.com/ps/products/185/ab185217/Images/ab185217-218428-anti-evpl-antibody-epr14688-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EVPL antibody [EPR14688] (ab185217)Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling EVPL with ab185217 at 1/1000 dilution. The slide is counterstained with Hematoxylin.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Flow Cytometry - Anti-EVPL antibody [EPR14688] (ab185217)](https://www.abcam.com/ps/products/185/ab185217/Images/ab185217-218427-anti-evpl-antibody-epr14688-flow-cytometry.jpg)
Flow Cytometry - Anti-EVPL antibody [EPR14688] (ab185217)Flow Cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling EVPL with ab185217 at 1/110 dilution (red) compared with a rabbit monoclonal IgG control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
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![Anti-EVPL antibody [EPR14688] (ab185217)](https://www.abcam.com/ps/products/185/ab185217/Images/ab185217-5-benefits-of-recombinant-antibodies.png)
Anti-EVPL antibody [EPR14688] (ab185217)
