Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ETS1 antibody [EPR21909] (ab220361)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21909] to ETS1
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ETS1 antibody [EPR21909]
    See all ETS1 primary antibodies

  • Description

    Rabbit monoclonal [EPR21909] to ETS1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human and mouse lymph node lysates; KARPAS-299 and C6 whole cell lysates; Rat lung and spleen lysates. IHC-P: Human tonsil and Hodgkin's lymphoma tissues; Mouse thymus tissue; Rat spleen tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ETS1 antibody [EPR21909] (ab220361)
    Western blot - Anti-ETS1 antibody [EPR21909] (ab220361)
    All lanes : Anti-ETS1 antibody [EPR21909] (ab220361) at 1/1000 dilution

    Lane 1 : KARPAS-299 (human anaplastic large cell lymphoma cell line) whole cell lysate at 20 µg
    Lane 2 : Rat lung lysate at 20 µg
    Lane 3 : Rat spleen lysate at 20 µg
    Lane 4 : C6 (rat glial tumor cell line) whole cell lysate at 10 µg
    Lane 5 : Mouse lymph node lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 50 kDa
    Observed band size: 50 kDa



    Exposure time : Lane 1: 125 seconds; Lanes 2,3 and 4: 3 minutes; Lane 5: 70 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate. This antibody has low affinity in WB. We suggest large amount of samples to be loaded or a high sensitive WB experiment, such as super-sensitive ECL substrate, to be used with this antibody.  

    Lower weak bands below 50 kDa represent other ETS1 isoforms.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (ab220361)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (ab220361)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling ETS1 with ab220361 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on human tonsil (PMID: 23288305; PMID: 10377039). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    We recommend that customers optimize their experimental conditions when testing on human spleen and thymus tissues.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-ETS1 antibody [EPR21909] (ab220361)
    Western blot - Anti-ETS1 antibody [EPR21909] (ab220361)
    Anti-ETS1 antibody [EPR21909] (ab220361) at 1/1000 dilution + Human lymph node lysate at 10 µg

    Secondary
    VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 50 kDa
    Observed band size: 50 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate. This antibody has low affinity in WB. We suggest large amount of samples to be loaded or a high sensitive WB experiment, such as super-sensitive ECL substrate, to be used with this antibody.  

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (ab220361)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (ab220361)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling ETS1 with ab220361 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on rat spleen (PMID: 23288305; PMID: 10377039). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (ab220361)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (ab220361)

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling ETS1 with ab220361 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on mouse thymus (PMID: 22307076; PMID: 3472202). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (ab220361)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETS1 antibody [EPR21909] (ab220361)

    Immunohistochemical analysis of paraffin-embedded human Hodgkin's lymphoma tissue labeling ETS1 with ab220361 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on human Hodgkin’s lymphoma (PMID: 23288305; PMID: 10377039). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    We recommend that customers optimize their experimental conditions when testing on human spleen and thymus tissues.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-ETS1 antibody [EPR21909] (ab220361)
    Anti-ETS1 antibody [EPR21909] (ab220361)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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