Anti-ERK5 antibody (ab196609)
Key features and details
- Rabbit polyclonal to ERK5
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-ERK5 antibody
See all ERK5 primary antibodies -
Description
Rabbit polyclonal to ERK5 -
Host species
Rabbit -
Specificity
ab196609 detects endogenous level of total ERK5 protein. -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment within Human ERK5 (N terminal). The exact sequence is proprietary.
Database link: Q13164 -
Positive control
- HeLa, K562, romas, and 293T cell lysates. Mouse brain and lung tissue lysates. Paraffin-embedded Human esophagus cancer tissue, paraffin-embedded rat kidney tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 49% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemical analysis of paraffin-embedded Human esophageal cancer tissue labeling ERK5 using ab196609 at 1/100 dilution.
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Anti-ERK5 antibody (ab196609) at 1/250 dilution + Human fetal muscle tissue at 40 µg
Secondary
Goat anti rabbit IgG at 1/8000 dilution
Predicted band size: 88 kDa
Exposure time: 40 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Human liver cancer tissue using ab196609 at dilution 1/20, the image on the right is treated with fusion protein.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Human lung cancer tissue using ab196609 at dilution 1/20, the image on the right is treated with fusion protein.
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Immunohistochemical analysis of paraffin-embedded Human esophagus labeling ERK5 using ab196609 at 1/100 dilution.
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Immunohistochemical analysis of paraffin-embedded rat kidney labeling ERK5 using ab196609 at 1/100 dilution.
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Immunofluorescence analysis of MCF7 cells labeling ERK5 usingab196609 at 1/50 dilution. Blue: DAPI for nuclear staining.