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Neuroscience Neurotransmission Intracellular Signaling Kinases

Anti-ERK5 antibody (ab196609)

Price and availability

284 784 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-ERK5 antibody (ab196609)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to ERK5
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-ERK5 antibody
    See all ERK5 primary antibodies
  • Description

    Rabbit polyclonal to ERK5
  • Host species

    Rabbit
  • Specificity

    ab196609 detects endogenous level of total ERK5 protein.
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment within Human ERK5 (N terminal). The exact sequence is proprietary.
    Database link: Q13164

  • Positive control

    • HeLa, K562, romas, and 293T cell lysates. Mouse brain and lung tissue lysates. Paraffin-embedded Human esophagus cancer tissue, paraffin-embedded rat kidney tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 49% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Intracellular Signaling
    • Kinases
    • Neuroscience
    • Neurology process
    • Neural Signal Transduction
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • MAPK Pathway
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK5 antibody (ab196609)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK5 antibody (ab196609)

    Immunohistochemical analysis of paraffin-embedded Human esophageal cancer tissue labeling ERK5 using ab196609 at 1/100 dilution.

  • Western blot - Anti-ERK5 antibody (ab196609)
    Western blot - Anti-ERK5 antibody (ab196609)
    Anti-ERK5 antibody (ab196609) at 1/250 dilution + Human fetal muscle tissue at 40 µg

    Secondary
    Goat anti rabbit IgG at 1/8000 dilution

    Predicted band size: 88 kDa


    Exposure time: 40 seconds
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK5 antibody (ab196609)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK5 antibody (ab196609)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Human liver cancer tissue using ab196609 at dilution 1/20, the image on the right is treated with fusion protein.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK5 antibody (ab196609)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK5 antibody (ab196609)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Human lung cancer tissue using ab196609 at dilution 1/20, the image on the right is treated with fusion protein.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK5 antibody (ab196609)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK5 antibody (ab196609)

    Immunohistochemical analysis of paraffin-embedded Human esophagus labeling ERK5 using ab196609 at 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK5 antibody (ab196609)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK5 antibody (ab196609)

    Immunohistochemical analysis of paraffin-embedded rat kidney labeling ERK5 using ab196609 at 1/100 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-ERK5 antibody (ab196609)
    Immunocytochemistry/ Immunofluorescence - Anti-ERK5 antibody (ab196609)

    Immunofluorescence analysis of MCF7 cells labeling ERK5 usingab196609 at 1/50 dilution. Blue: DAPI for nuclear staining.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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