Anti-ERK1 (phospho Y204) antibody (ab131438)
Key features and details
- Rabbit polyclonal to ERK1 (phospho Y204)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ERK1 (phospho Y204) antibody
See all ERK1 primary antibodies -
Description
Rabbit polyclonal to ERK1 (phospho Y204) -
Host species
Rabbit -
Specificity
ab131438 detects endogenous levels of ERK1 only when phosphorylated at tyrosine 204. -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human ERK1 aa 202-206 (phospho Y204) conjugated to Keyhole Limpet Haemocyanin (KLH).
Sequence:T-E-Y(p)-V-A
Database link: P27361 -
Positive control
- 293 cell extract untreated or treated with UV; HeLa cells; Human breast carcinoma tissue
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab131438 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-ERK1 (phospho Y204) antibody (ab131438) at 1/500 dilution
Lane 1 : 293 cell extract untreated
Lane 2 : 293 cell extract treated with UV
Predicted band size: 43 kDa
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Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling ERK1 (phospho Y204) with ab131438 at 1/50 dilution. Right panel was preincubated with blocking peptide.
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Immunofluorescent analysis of methanol-fixed HeLa cells labelling ERK1 (phospho Y204) with ab131438 at 1/100 dilution.